Mammalian Cell PE LB™ has been developed for extraction of total soluble proteins from mammalian cultured cells. The Mammalian Cell PE LB™ is based on organic buffering agents, which utilizes a mild non-ionic detergent, and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. Depending on the application, additional agents such as chelating agents, reducing agents and protease inhibitors may be added into Mammalian Cell PE LB™. Mammalian Cell PE LB™ reagent has been tested for use with a wide variety of mammalian cells. Mammalian Cell PE LB™ can be used for both suspension as well as adherent cells. The proprietary combination of this reagent provides a simple and versatile method for the extraction of proteins from mammalian cells.
COMPATIBILITY
Mammalian Cell PE LB™ is compatible with most applications, including enzyme assays, various chromatography procedures, electrophoresis, etc. Mammalian Cell PE LB™ is also compatible for protein estimation with NI™ protein assay (Non-Interfering Protein Assay™). The protein extract prepared with Mammalian Cell PE LB™ may be used for most enzyme assays including reporter gene assays (e.g. β-galactosidase, luciferase, chloramphenicol acetyltransferase), kinases (e.g., PKC, PKA, Tyrosine Kinase), and immunoassays (e.g., ELISA, Western blots, RIA).
Features
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Provides a simple and versatile method for protein extraction from mammalian cells
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Compatible with electrophoresis and chromatographic applications
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Compatible with most enzyme assays including:
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Reporter gene assays; ß-galactosidase, luciferase, chloramphenicol acetyltransferase
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Kinases; Protein kinase C, protein kinase A, tyrosine kinase
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Immunoassays; ELISA, Western blots, RIA
Applications
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For extraction of soluble proteins from adherent and suspension animal cultured cells
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Suitable for most applications including enzyme and protein purification applications, electrophoresis, Western blotting and 2D-gel analysis
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