Plasmid Purification & Colony Screening

Plasmid Isolation

Spin column format miniprep plasmid isolation kits from bacteria available are GET™ Plasmid DNA Miniprep for up to 20µg DNA from 1-5ml culture and a 96-well plate format, GET™ Plasmid DNA 96-Well. Select the EZ Yeast™ Plasmid Prep kit for yeast plasmid purification.

Alternatively, our Silica Magentic Beads can be used for the purification of plasmid DNA following alkaline lysis.

Plasmid Transformation & Transfection

 Z-Competent™ E. coli Transformation kit is designed to generate competent E. coli cells for simple and highly efficient E. coli transformation.

G-FECT™ DNA Transfection reagents are a series of powerful DNA transfection reagents for the effective and reproducible transfection of DNA into mammalian cells.  G-FECT™ can be used for plasmid transfection, genome editing, virus production and more. 

Colony Screening

A large selection of antibiotics are offered for colony selection, including HP Ampicillin™, a stronger and more stable inhibitor of ß-lactamase than regular ampicillin.

For blue/white colony screening, a molecular biology grade IPTG is available. 

For rapid colony screening, including PCR-colony screening, we recommend our Plasmid Screening ToothPick™ or ToothPick™-PCR for the rapid analysis of single colonies by restriction digestion or PCR. No need for an overnight culture.