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ß-Galactosidase Assays
β-galactosidase, also called beta-gal or β-gal, is a hydrolase enzyme that catalyzes the hydrolysis of β-galactosides into monosaccharides. Substrates of different β-galactosidases include ganglioside GM1, lactosylceramides, lactose, and various glycoproteins. The β-galactosidase gene functions well as a reporter gene for two major reasons: its protein product is extremely stable and resistant to proteolytic degradation in cellular lysates, and most importantly, the enzyme is easily assayed.
G-Biosciences offers highly sensitive colorimetric and fluorescent assays for the reporter gene β-galactosidase.