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Labeling & Conjugation

G-Biosciences offer a vast array of products for protein labeling and conjugations. Both fluorescent and biotin labeling kits are available as well as products for agarose conjugation and accessories. 

Fluorescent dyes have become a common tool in laboratories for the visualization and studying of proteins, whether they are attached directly to the protein of interest or to antibodies specific for the protein of interest. 

G-Biosciences researchers have developed two kits for the rapid and efficient labeling of proteins, including antibodies, with a fluorescent green (FITC) or red ((5/6) TAMRA-SE) dye.  Each kit is supplied with five of our single-use OneQuant™ fluorescent reagents that protect the dyes from exposure to light and minimize hands-on time.  The following evaluates labeling of two different proteins with fluorescent dyes.

 

HOOK-Biotin kits come with all the necessary reagents and equipment for optimization of reaction conditions, efficient labeling, removal of unbound biotin and quantification of biotin labeling.  In addition to highly efficient labeling, the HOOK-Biotin kits offer the advantage of being supplied with micro dialysis units and a specific Optimizer Buffer.  These greatly simplify the labeling process and ensure high levels of biotin labeling.

 

Download the Protein Labeling & Conjugation Handbook

Avidin is a glycoprotein with approximately 10% of its total mass comes from carbohydrates. Avidin has a molecular weight of 67kDa and contains four i..
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An important tool in research is the generation of peptide and protein affinity columns for the purification of antibodies and for the discovery of im..
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HOOK™ BiotinQuant™ uses HABA (2-(4-Hydroxyphenylazo)benzoic acid/ 2-(4′-Hydroxybenzeneazo)benzoic acid/ 4′-Hydroxyazobenzene-2..
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HOOK™ Cell Surface Protein Isolation kit uses G-Biosciences HOOK™ biotin labeling and purification technology in conjunction with our Mamm..
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(5/6) TAMRA-SE (5-(and-6)-Carboxytetramethylrhodamine succinimidyl ester, mixed isomers) is based on tetramethylrhodamine, one of the most common fluo..
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FITC (fluorescein isothiocyanate) is a commonly used fluorescent label for proteins, as it contains the groups required for conjugating to amino, sulf..
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HOOK™ Biotin-BMMCC reacts with protein free sulhydryl groups. Sulfhydryl reactive reagents are more specific and react only with free sulfhydryl..
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The use of biotin for non-radioactive labeling of proteins and nucleic acids has now become an increasingly popular technique in life science research..
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The use of biotin for non-radioactive labeling of proteins and nucleic acids has now become an increasingly popular technique in life science research..
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HOOK™ Biotin-PDA reacts with protein free sulhydryl groups. Sulfhydryl reactive reagents are more specific and react only with free sulfhydryl r..
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The use of biotin for non-radioactive labeling of proteins and nucleic acids has become an increasingly popular technique in life science re..
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The use of biotin for non-radioactive labeling of proteins and nucleic acids has become an increasingly popular technique in life science research. Se..
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HOOK™ Iodoacetyl-LC-Biotin reacts with protein free sulhydryl groups. Sulfhydryl reactive reagents are more specific and react only with free su..
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HOOK™ NHS-Biotin reacts with protein primary amines. Amines, lysine ε-amines and N-terminal α-amines, are the most abundant group ..
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