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Reducing Reagents

Download the Crosslinkers Handbook & Selection Guide            Download the Protein Labeling & Conjugation Handbook

 

The reduction of disulfide bridges in protein is a routinely used technique in proteomics and is involved in protein denaturation, solubilization, protection of protein thiols, characterization of protein (disulfide bonds), separation and study of protein subunits, and protein activation for cross-linking.

Numerous protein reductants, including DTT, ß-mercaptoethanol and TCEP (Tris (2-carboxyethyl) phosphine) are offered in different formats, including powders, solutions and OneQuant™ formats.

1,4 Dithiothreitol (DTT) is a small molecule reducing agent, also known as Cleland’s reagent. G-Biosciences DTT is a high quality proteomic grade DTT and is supplied in various sizes, including a OneQuant™ version. The OneQuant™ format features 40 vials of 7.7mg DTT for conven..
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5,5'-dithio-bis-(2-nitrobenzoic acid) (DTNB)   Ellman's reagent is a versatile, water-soluble compound for quantifying free sulfhydryl groups in solution. It reacts with a free sulfhydryl group to yield a mixed disulfide and 2-nitro-5-thiobenzoic acid (NTB), a measurable yellow colore..
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Hydroxylamine•HCl is a reducing agent that is routinely used for the deacetylation of SATA to form free sulfhydryls (Figure 1), for cleavage of protein cross-linkers that contain carbonyl groups (i.e. EGS) and for mutagenesis of plasmid DNA.   Features Synonym: Hydroxylammonium chlor..
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Reducing agents are used in the reduction of disulfide bonds of proteins and peptides.  It is often necessary to remove the reducing agents from the protein/peptide solutions to prevent them from interfering with subsequent procedures.  However, for small proteins, and particularly peptide..
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A water soluble, odorless, non-toxic and stable protein reductant. Protein-S-S-Reductant™ uses TCEP (Tris [2-carboxyethyl] phosphine), a popular alternative to ß-mercaptoethanol and DTT (dithiothreitol). TCEP improves stability, increases effectiveness, and reduces proteins over a wider ..
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Carbonyl groups, including aldehydes, ketones and glyoxals, react with amines to form Schiff base intermediates that are in equilibrium with their free forms.  The labile Schiff’s base interaction can be stabilized by chemical reduction. If the Schiff’s base is formed between an ald..
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TCEP is a water-soluble, odorless, non-toxic and stable protein reductant. This potent reducing agent is a popular alternative to ß-mercaptoethanol and DTT (dithiothreitol) use for most applications. The reduction potency of TCEP is twice as high as that of DTT, and TCEP is effective in reduci..
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High quality proteomic grade β-mercaptoethanol.   β-mercaptoethanol, a popular reducing agent, is offered in 5ml and 100ml bottles.   Features Synonym: β-Mercaptoethanol CAS#: 60-24-2 Molecular Formula: C2H6OS Molecular Weight: 78.13  ..
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