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Western Blotting

Download the Western Blotting Handbook for Troubleshooting Tips

 

Transfer systems, chemiluminescence and chromogenic detection systems, blocking and wash buffers, antibodies and enzyme labelling kits.

The Blocking Agents Trial Packs are a set of four G-Biosciences proprietary blocking agents for immunoassays with one being a non-animal protein blocker and another protein free that minimizes the risk of non-specific binding of antibodies during the immunoassay. Each blocking buffer provided in the..
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A unique stain for reversibly staining protein on nitrocellulose and PVDF transfer membranes.   BLOT-FastStain™ only stains protein and leaves the background absolutely untouched and brilliant white resulting in high band visibility. The staining procedure takes 10 minutes and has a sen..
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G-Biosciences scientists have developed a high efficiency transfer buffer for Western blotting. The pre-made and ready-to-use [20X] transfer buffer is prepared for optimal conductivity and efficient protein transfer without generating excessive heat or transfer distortion. Efficient™ Western T..
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femto TBST™ and femto PBST™ will enhance sensitivity of your immunoassays by minimizing the "washing out" of immuno agents or immuno complexes, a common problem associated with classical TBST and PBST buffers. The wash buffers aid in the generation of cleaner backgrounds, resul..
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femtoCHROMO™ AP kit is supplied with a single step chromogenic substrate for the rapid development of protein bands with low background staining. To ensure low background staining, each kit is supplied with an enhanced blocking agent, BLOT-QuickBlocker™, and a concentrated (10X) was..
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femtoCHROMO™-HRP kit is supplied with a single step chromogenic substrate for the rapid development of protein bands with low background staining.  To ensure low background staining, each kit is supplied with an enhanced blocking agent, BLOT-QuickBlocker™, and a concentrated (10X) w..
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The femtoLUCENT™ PLUS-AP kit is based on alkaline phosphatase (AP) chemiluminescence reaction. The chemiluminescence light emission can be recorded by a short exposure to blue-light sensitive autoradiography films. The femtoLUCENT™ PLUS-AP kit allows for detection of femtogram quantity o..
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FirstChoice™ Blocking Buffers contain a proprietary protein formulation that offers greater versatility and lack of cross-reactivity.  This makes FirstChoice™ Blocking Buffers ideal as a first choice for optimization of new assays, system or when determining the optimal blocking buf..
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FISH-Blocker is a blocking agent that uses a fish protein as the primary blocking agent.  The use of a fish protein, a non-mammalian protein, is that it eliminates or minimizes the interaction of antibodies raised in mammals.  FISH-Blocker is one of the best blocking agents for immunoassay..
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For the highly sensitive detection of glycoproteins following gel electrophoresis (Figure 1) or protein transfer to nitrocellulose membranes (Figure 2).   The kit uses an enhanced Periodic Acid-Schiff (PAS) method for detection of glycoprotein sugars.  The supplied oxidizing agent first ..
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Based on Efficient™ Western Transfer Buffer, the High Molecular Weight Transfer buffer is designed to facilitate the transfer of notoriously difficult high molecular weight proteins (>70kDa) during Western blotting.   Supplied as 1 liter of a 5X concentrated solution...
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For user's convenience, we offer pre-cut transfer membranes and padding for Western blot transfer procedures. Pre-cut membranes are supplied sandwiched between blotting paper padding. Simply soak the membrane in transfer buffer and assemble with the gel in a transfer cassette. Nitrocellulose, (0..
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