Methylation of key biological molecules and proteins plays important roles in numerous biological systems, including signal transduction, biosynthesis, protein repair, gene silencing and chromatin regulation (1).
The S-adenosylmethionine (SAM) dependent methyltransferases use SAM, the second most commonly used enzymatic cofactor after ATP. SAM, also known as AdoMet, acts as a donor of a methyl group that is required for the modification of proteins and DNA. Aberrant levels of SAM have been linked to many abnormalities, including Alzheimer’s, depression, Parkinson’s, multiple sclerosis, liver failure and cancer (2).
The G-Biosciences’ SAM Methyltransferase Assays are continuous enzyme coupled assays that can continuously monitor SAM-dependent methyltransferases (3) without the use of radioactive labels or endpoint measurements. A sensitive, UV-based, SAM265, and a colorimetric, SAM510, SAM Methyltransferase assays are offered. The fluorescent SAMfluoro: (SAM) S-Adenosyl Methyltransferase Assay is also available.
1. Cheng, X. & Blumenthal, R.M. (1999) S-Adenosylmethionine Dependent Methyltransferases World Scientific, Singapore.
2. Schubert, H.L. et al. (2003) Trends Biochem. Sci 28: 329-335.
3. Dorgan, K.M. et al. (2006) Anal. Biochem. 350:249-255.
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