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The rAmylase Project

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The rAmylase Project is a unique set of ten self-contained laboratory experiments, kitted for teacher convenience. With The rAmylase Project Kits it is easy to pull together a unit, a semester, or a year-long course in biotechnology.

The kits can be used individually in a "plug'n'play" fashion to teach specific biotechnology techniques or, as a thematic unit that demonstrates how a commercially important biotechnology product, the amylase protein, is researched and manufactured. The rAmylase Project kits demonstrate that DNA is the flash but proteins are the cash of biotechnology.

The rAmylase Project Kits
  1. Characterizing Proteins by PAGE
  2. Assaying for Amylase Activity
  3. Direct ELISA of Bacterial α-Amylase
  4. Western Blot to Identify α-Amylase
  5. Determining the Concentration of Amylase in a Solution
  6. Restriction Digestion to Verify the pAmylase Plasmid
  7. Transformation of E. coli with pAmylase
  8. Using A Miniprep Kit for Plasmid Isolation
  9. Using Ion Exchange Chromatography to Separate Proteins
  10. Confirming the Amylase Gene Using PCR

  

This kit is designed to teach 8 groups of four students the principles of enzyme assays.   The kit contains the reagents to allow students to test for the presence and activity of 2 different types of amylase by: Visualizing the breakdown of the starch substrate by the amylase enzyme using..
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This kit is designed to teach 8 groups of four students the principles of Protein Electrophoresis. The kit contains the reagents to allow students to: Conduct a serial dilution of a stock amylase solution Prepare samples for PAGE electrophoresis Separate and distinguish proteins by electrophor..
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This kit is designed to teach 8 groups of four students the principles of the polymerase chain reaction (PCR)   The kit contains the reagents to allow students to: Set up multiple PCR reactions using pAmylase plasmid DNA and bacterial genomic DNA templates Set up a PCR negative control&nb..
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This kit is designed to teach 8 groups of four students the principles of protein concentration estimation assays. The kit contains the reagents to allow students to: Conduct a serial dilution of a stock amylase solution to create standards of known concentration Use a Bradford protein assay to ..
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This kit is designed to teach 8 groups of four students the principles of ELISA (Enzyme Linked ImmunoSorbent Assays).   The kit contains the reagents to allow students to: Conduct a serial dilution of a stock amylase solution to create standards of known concentration Bind known and unkno..
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This kit is designed to teach 8 groups of four students the principles of restriction digestion and agarose electrophoresis. The kit contains the reagents to allow students to: Set up restriction digests of the p-Amylase plasmid with two enzymes Set up positive and negative controls for the rest..
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This kit is designed to teach 8 groups of four students the principles of bacterial transformation.   The kit contains the reagents to allow students to: Generate E.coli comptent cells Transform competent cells with the p-Amylase plasmid Grow transformed colonies on selection plates of L..
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This kit is designed to teach 8 groups of four students the principles of plasmid DNA purification by minipreps.   The kit contains the reagents to allow students to: Collect p-Amylase-transformed bacteria from an overnight culture Resuspend bacteria, lyse and isolate the p-Amylase plasmi..
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This kit is designed to teach 8 groups of four students the principles of Western blotting.   The kit contains the reagents to allow students to: Conduct a serial dilution of a stock amylase solution Prepare samples for PAGE electrophoresis Separate proteins by electrophoresis Transfer ..
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This kit is designed to teach 8 groups of four students the principles of Ion Exchange Chromatography and protein purification.  The kit contains the reagents to allow students to: Equilibrate a DEAE agarose column Load a protein sample on the column (amylase, lysozyme or a mixture of both)..
$0.00
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