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Oxidative Stress

Antioxidant capacity is an overall ability of organisms or food to catch free radicals and prevent their harmful effect.

G-Biosciences Antioxidant Capacity Assays are useful for the determination of this parameter in different sample types such as food and beverages or plasma.

There are two types of mechanisms to determine the antioxidant capacity on different matrices: electron-transfer (E-T) methods, which measure the ability of an antioxidant to reduce an oxidant, with a change of color (ABTS, FRAP, DMPD) and hydrogen atom mediated (HAT) methods, which measure the ability of an antioxidant to eliminate free radicals through the donation of a hydrogen atom (they normally quantify peroxyl radicals; ORAC).

Antioxidant capacity is an overall ability of organisms or food to catch free radicals and prevent their harmful effect. Antioxidative effect includes protection of cells and cellular structures against harmful effect of free radicals, especially oxygen and nitrogen. Substances with antioxidative pr..
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Catalase is an enzyme present in blood and other tissues with antioxidant activity. This enzyme can catalyze the reaction that consumes hydrogen peroxide, transforming it into water and oxygen. Since hydrogen peroxide is difficult to be measured directly in biological samples, the determination of t..
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Catalase, an antioxidant enzyme, is present universally in all mammalian and non-mammalian aerobic cells, which contain a cytochrome system. Catalase is involved in the removal of hydrogen peroxide, a reactive oxygen species (ROS) which is toxic to the cells. It is produced both during normal aerobi..
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Free radicals are highly reactive species that are naturally formed during cell metabolism in the mitochondria, secretion by immune cells during inflammatory processes or taken up as environmental pollutants. CUPRAC (CUPric Reducing Antioxidant Capacity) Assay is a new method, which enables the To..
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Antioxidant capacity is an overall ability of organisms or food to catch free radicals and prevent their harmful effect. Antioxidative effect includes protection of cells and cellular structures against harmful effect of free radicals, especially oxygen and nitrogen. Substances with antioxidative pr..
$0.00
Antioxidant capacity is an overall ability of organisms or food to catch free radicals and prevent their harmful effect. Antioxidative effect includes protection of cells and cellular structures against harmful effect of free radicals, especially oxygen and nitrogen. Substances with antioxidative pr..
$0.00
Lipid peroxidation is a well-known example of oxidative damage in cell membranes, lipoproteins, and other lipid-containing structures. Peroxidative modification of unsaturated phospholipids, glycolipids, and cholesterol can occur in reactions triggered by i) free radical species such as oxyl radical..
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Malondialdehyde (MDA), can be generated by oxidizing agents that alters lipid structure, creating lipid peroxides. MDA can be measured as Thiobarbituric Acid Reactive Substances (TBARS). This method, is convenient to determine the relative lipid peroxide content of samples, including plasma, serum,..
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Nitric oxide is an important molecular messenger in the vascular and nervous systems. It has multiple physiological roles, such as vasorelaxation or neuronal signaling, but it also has other complex pathophysiological effects. It is synthesized by the three isoforms of the nitric oxide synthases (eN..
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Nitric oxide is an important molecular messenger in the vascular and nervous systems. It has multiple physiological roles, such as vasorelaxation or neuronal signaling, but it also has other complex pathophysiological effects. It is synthesized by the three isoforms of the nitric oxide synthases (eN..
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Model:
G-Biosciences’ ORAC assay depends on the free radical damage to a fluorescent probe, such as fluorescein, to result in a change of fluorescent intensity and the degree of change is indicative of the amount of radical damage. The presence of antioxidants results in an inhibition in the free rad..
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PEROXsay™ is a colorimetric quantitative peroxide assay that measures the oxidation of ferrous (Fe2+) ions to ferric (Fe3+) ions. Peroxides react with a sugar alcohol converting it to a peroxyl radical that subsequently starts the oxidation of ferrous ions to ferric ions. The acidic pH of the ..
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