Hot Start Taq DNA Polymerase

G-biosciences hot start Taq DNA Polymerase is chemically modified that possesses a 5´→3´ polymerase activity and a 5´-flap endonuclease activity. Hot start Taq DNA Polymerase reduces non-specific amplification during PCR setup and increases PCR specificity and sensitivity.

Product Source:

• E. coli strain expressing a Taq DNA Polymerase gene from Thermus aquaticus.

Product Includes:

• Hot start Taq DNA Polymerase
• 10X PCR Buffer with Mg²⁺

Unit Definition:

One unit is defined as the amount of enzyme that incorporates 10 nmoles of dNTP into acid-insoluble form in 30 minutes at 72º C.

10X PCR buffer with Mg²⁺:

100 mM Tris-HCl pH 9.0, 15 mM MgCl₂, 100 mM KCl, 80 mM (NH₄)₂SO4, 0.5% Igepal CA 630, 0.5% Tween 20, 50% Glycerol

Storage Buffer:

50 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 7.5 @ 25ºC

Storage Temperature:

-20ºC