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Sample Preparation

Download the Protein Sample Preparation Handbook

 

Sample preparation is a key procedure in sample analysis.  Frequently, the component of interest is present in levels too low for detection or the presence of interfering matrix elements can mask the analysis of the component of interest. Sample preparation can concentrate the component to adequate levels for measurement and remove excess contaminants to yield clean, informative samples. G-Biosciences offers a variety of products for sample preparation.

Dialysis is a popular technique used for the exchange of buffer medium across semi-permeable membranes. Dialysis devices are available in many configurations for research applications. We offer innovative dialysis devices and accessories for processing small samples. Tube-O-Array is available for the dialysis of up to 12 samples at one time.

Many research applications require concentration of dilute protein solutions. Protein solutions may be concentrated by precipitation, chromatographic capture and elution, or by lyophilization. G-Biosciences offers a selection of protein concentration systems suitable for concentrating proteins for running gels, raising antibodies, protein purification, protein assays and other applications.  Proteins concentrated with these kits are suitable for the majority of downstream applications.

For removing contaminants such as detergents, salts, dyes, and radioisotopes we offer a selection of contamination removal systems. These include our ready-to-use disposable desalting columns and Spin-OUT Columns for removing contamination from protein and nucleic acid samples.

Sputo-LR™ is a sputum liquefying reagent for sputum and other mucoid fluids that is useful for the isolation of pathogenic or saprophytic bacteria, fungi, yeast, and epithelial cells. Sputo-LR™ is a concentrated sterile solution of Cleland’s Reagent (dithiothreitol, DTT) in phospha..
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Tube-O-CONCENTRATOR™ is a versatile concentration device that utilizes a novel, water absorbing, liquid polymer and our patented Tube-O-DIALYZER™ for the rapid concentration of dilute protein solutions with zero protein loss.  The unique tube design of Tube-O-DIALYZER™ ensures..
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Tube-O-Reactor™ is a system that allows all the key steps of cross linking, coupling and modification of proteins and/or nucleic acids to be performed in a single tube. This minimizes the risk of sample loss, of experimental time and of hands-on phases.   Most of the above reactions inv..
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Our Universal Protein Precipitation Agent, commonly known as UPPA™, has been specifically designed for the quantitative concentration of protein solutions as dilute as 1ng/ml into a small volume. Protein concentration and precipitation is not affected by the presence of detergents, chaotropics..
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Protocols.io provides an interactive version of this protocol where you can discover and share optimizations with the research community.    The UPPA-PROTEIN Concentrate™ kit uses our proprietary reagent UPPA™ (Universal Protein Precipitation Agent) to quantitatively concentr..
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Model:
The SpinOUT™ G-Acryl columns are versatile, spin-format columns for the desalting and buffer exchange of peptide and protein and other macromolecule solutions ranging from 5µl through to 3ml sample volumes.  The SpinOUT™ columns are available in three MWCO sizes for >1,800,..
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Model:
Protocols.io provides an interactive version of this protocol where you can discover and share optimizations with the research community.    Dialysis is a popular technique used for the exchange of buffer medium across semi-permeable membranes. Dialysis devices are available in many ..
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