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Sample Preparation

Download the Protein Sample Preparation Handbook

 

Sample preparation is a key procedure in sample analysis.  Frequently, the component of interest is present in levels too low for detection or the presence of interfering matrix elements can mask the analysis of the component of interest. Sample preparation can concentrate the component to adequate levels for measurement and remove excess contaminants to yield clean, informative samples. G-Biosciences offers a variety of products for sample preparation.

Dialysis is a popular technique used for the exchange of buffer medium across semi-permeable membranes. Dialysis devices are available in many configurations for research applications. We offer innovative dialysis devices and accessories for processing small samples. Tube-O-Array is available for the dialysis of up to 12 samples at one time.

Many research applications require concentration of dilute protein solutions. Protein solutions may be concentrated by precipitation, chromatographic capture and elution, or by lyophilization. G-Biosciences offers a selection of protein concentration systems suitable for concentrating proteins for running gels, raising antibodies, protein purification, protein assays and other applications.  Proteins concentrated with these kits are suitable for the majority of downstream applications.

For removing contaminants such as detergents, salts, dyes, and radioisotopes we offer a selection of contamination removal systems. These include our ready-to-use disposable desalting columns and Spin-OUT Columns for removing contamination from protein and nucleic acid samples.

G-Biosciences C18 Spin Columns are ready-to-use micro centrifuge columns for peptide clean up and concentration.  The columns consist of porous C18 reverse-phase resin that has a particle size if ~15µm and a pore size of 300Å.  The resin offers highly efficient binding and reco..
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The Column-PROTEIN-Concentrate™ kit has been specifically developed for concentration of those proteins that cannot be concentrated either by precipitation or other techniques. The kit is based on a proprietary Protein Binding Resin that binds and immobilizes any protein in a low salt buffer b..
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Concentrator Powder is a high molecular weight polymer which will not migrate across the dialysis membrane.  Simply transfer your dilute protein solution to a dialysis bag or dialysis device, such as our patented Tube-O-DIALYZER™ and then cover the membrane with Concentrator Powder. ..
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Concentrator Solution is a novel liquid polymer for the rapid concentration of dilute protein solutions with zero loss, using dialysis.   Simply transfer your dilute protein solution to a dialysis bag or dialysis device*, such as our patented Tube-O-DIALYZER™ and dialyze against the con..
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DeSalting Columns are ready-to-use disposable columns for separating macromolecules, i.e. proteins, from low molecular weight buffer salts and reagents. See our SpinOUT™ product line for rapid desalting of proteins >6 or >30kDa...
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Dialysis is the process of separating molecules in solution by the difference in their rates of diffusion through a semi permeable membrane, such as dialysis tubing or Tube-O-DIALYZER™ dialysis caps. Molecules small enough to pass through the dialysis membrane move across the membrane in ..
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EndotoxinOUT™ was designed for the rapid removal of endotoxins and pyrogens (LPS, lipopolysaccharides) from nucleic acid (DNA) samples. EndotoxinOUT™ is an ideal product for the cleanup of buffers, cell culture media, protein solutions, nucleic acid (DNA) samples and pharmacological comp..
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G-Trap™ GT-1200 Desalting Column is prepacked ready to use column for separation of low molecular weight substances from high molecular weight substances based on size exclusion chromatography.    G-Trap™ GT-1200 Desalting Columns are mostly used for removal of salt or buffer..
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The OrgoSol-PROTEIN-Concentration™ kit precipitates protein with a proprietary solvent buffer, OrgoSol™. The OrgoSol™ buffer has been specifically developed for efficient precipitation of protein solutions with minimal disruption to the protein structure and therefore maintains the..
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PAGE-Optimizer™ is a unique, spin column for the preparation of samples for SDS-PAGE.  The columns contain a proprietary separation matrix that removes contaminants such as salts, detergents, cellular agents and other common buffering agents.     PAGE-Optimizer™ helps el..
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Remove Electrophoretic Contaminants for Improved Protein Resolution and Publication Quality Gels; Ideal for Difficult or Dilute Samples   Many lysis buffers and reagents used in sample preparation are incompatible with routinely used electrophoretic analysis. The presence of contaminants, or ..
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Protocols.io provides an interactive version of this protocol where you can discover and share optimizations with the research community.    The SpinOUT™ GT-100, GT-600 and GT-1200 columns are versatile, spin-format columns for the desalting and buffer exchange of peptide, protein ..
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