Bioassays
Our Bioassays cover a wide range of proteomic and cell biology fields. Our CytoScan™ products offer a range of
non-radioactive bioassays for the study of chemical and cell-mediated cytotoxicity, viability, cell death or cell proliferation. Included are cell proliferation and cell toxicity assays which feature lactate dehydrogenase (LDH) assays, WST-1 assays and sulforhodamine B (SRB) assays. Our apoptotic line features caspase assays and a wide selection of inhibitors.
We offer continuous enzyme coupled assays for methylation that can continuously monitor SAM-dependent methyltransferases without the use of radioactive labels or endpoint measurements.
We have a phosphatase assay for the quantification of phosphatases and three phosphatase inhibitor cocktails to inhibit phosphatases and for phosphatase studies.
PhosphoQuant™ is specifically designed for quick and reliable determination of whether a purified protein is phosphorylated and the extent of phosphorylation. The assay is based on the alkaline hydrolysis of phosphates from seryl and threonyl residues in phosphoproteins and the subsequent quantifica..
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-500x500h.jpg "Protease Assay Kit")
Gene/Protein:
The Protease Activity Assay Kit is designed for the quantitative determination of proteases present in a protein sample, using a dye-labeled protein substrate.
The proteases present in the sample of interest will digest the protein substrate and release dye labeled peptides. The absorbance of the..
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-500x500h.jpg "Protease Assay Substrates")
Gene/Protein:
RESORUFIN-CASEIN PROTEASE SUBSTRATE
A colorimetric substrate that when treated with proteases releases resorufin that has an absorbance of 570nm. Supplied lyophilized.
FITC-CASEIN PROTEASE SUBSTRATE
A fluorescent (fluorescein isothiocyanate (FITC)) substrate that when ..
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Sensitive protease screening is needed to study protease activity present in the sample of interest. The Protease Screening Kit provides you with a simple and quick method for testing your samples for proteolysis.
Simply incubate your sample in the reagent provided and obtain results. The screening..
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Gene/Protein:
Rapidly, mix 1µl protein sample with the supplied Dilution Buffer and apply 1µl of the solution to the test strip by point-of-contact capillary action. Under the assay's specific buffer conditions the protein enters into the matrix of the test strip, binds and saturates as protein di..
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Protocols.io provides an interactive version of this protocol where you can discover and share optimizations with the research community.
ProteSEEKER™ identifies specific types of proteases with a panel of twelve protease inhibitors and a sensitive colorimetr..
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Protn-Latex™ is a simple and reliable method for estimation of protein contamination in latex. Protein estimation can be performed either on the production site or in any laboratory with a minimum of skill or instrumentation. The test detects as low as 25-40µg protein /gm latex (or 25-50..
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Recombinant human caspases 1-3 and 6-10 are supplied lyophilized in quantities of 25 units. One unit of the specific recombinant caspase is the enzyme activity that cleaves 1nmol of a specific caspase substrate per hour at 37ºC in a reaction solution containing 50mM HEPES (pH7.2), 50mM..
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Methylation of key biological molecules and proteins plays important roles in numerous biological systems, including signal transduction, biosynthesis, protein repair, gene silencing and chromatin regulation (1). The S-adenosylmethionine (SAM) dependent methyltransferases use SAM, the..
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Gene/Protein:
Trypan Blue 0.4% Solution is commonly used as a cell stain to measure cell viability using dye exclusion method. The dye exclusion method is based on the principle that cell impermeable dyes like Trypan blue will stain only dead cells where as viable cells will not be stained.
Trypan Blue stainin..
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Gene/Protein:
β-Galactosidase Assay (CPRG) provides an easy, rapid, and highly sensitive method for determining the β-galactosidase activity in the lysates of cells transfected with a β-galactosidase expression construct. The β-Galactosidase Assay (CPRG) assay uses chlorophenol red-&beta..
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-500x500h.jpg "CB-X™ Protein Assay")
Gene/Protein:
Protocols.io provides an interactive version of this protocol where you can discover and share optimizations with the research community.
Protein assays are routinely used in many research fields to estimate proteins in a vast array of buffers and conditions. A major pr..
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