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Bioassays

Download the Bioassay Handbook & Selection Guide for Complete Product Listing

 

Our Bioassays cover a wide range of proteomic and cell biology fields. Our CytoScan products offer a range of
non-radioactive bioassays for the study of chemical and cell-mediated cytotoxicity, viability, cell death or cell proliferation. Included are cell proliferation and cell toxicity assays which feature lactate dehydrogenase (LDH) assays, WST-1 assays and  sulforhodamine B (SRB) assays. Our apoptotic line features caspase assays and a wide selection of inhibitors.

 
An array of highly sensitive protein quantification assays are available including NI(Non-Interfering) protein assay, a colorimetric protein assay that overcomes interference of common laboratory agents, and our CB-X  assay which is designed to be compatible with all commonly used buffers and conditions.

 

We offer continuous enzyme coupled assays for methylation that can continuously monitor SAM-dependent methyltransferases without the use of radioactive labels or endpoint measurements.

 

We have a phosphatase assay for the quantification of phosphatases and three phosphatase inhibitor cocktails to inhibit phosphatases and for phosphatase studies.

 
PEROXsay Peroxide Assays and a variety of enzymes are also available.
Firefly Luciferase Assays are one of the widely used reporter assays to detect and measure gene expression, function and regulation.  G-Biosciences Lumino™ Firefly Luciferase Assay is designed for rapid and high sensitive detection or quantification of firefly luciferase reporter enzyme ..
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Lumino™ Firefly Luciferase Glow Assay is designed for high throughput screening or quantification of luciferase expression in mammalian cells which is mainly performed by batch processing of 96- and 384- well plates. Lumino™ Firefly Luciferase Glow Assay is homogenous, high-sensitive, g..
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The Phosphatase Assay kit is designed to measure the activity of phosphatases in biological samples and to screen for agonists and inhibitors of phosphatases.   The Phosphatase Assay kit uses para-nitrophenyl phosphate (pNPP), a chromogenic substrate for most phosphatases, including alkaline ..
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PhosphoQuant™ is specifically designed for quick and reliable determination of whether a purified protein is phosphorylated and the extent of phosphorylation. The assay is based on the alkaline hydrolysis of phosphates from seryl and threonyl residues in phosphoproteins and the subsequent quantifica..
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Gene/Protein:
The Protease Assay Kit is designed for the quantitative determination of proteases present in a protein sample, using a dye-labeled protein substrate. The proteases present in the sample of interest will digest the protein substrate and release dye labeled peptides. The absorbance of the dye-labe..
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Gene/Protein:
RESORUFIN-CASEIN PROTEASE SUBSTRATE A colorimetric substrate that when treated with proteases releases resorufin that has an absorbance of 570nm. Supplied lyophilized.   FITC-CASEIN PROTEASE SUBSTRATE A fluorescent (fluorescein isothiocyanate (FITC)) substrate that when ..
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Sensitive protease screening is needed to study protease activity present in the sample of interest. The Protease Screening Kit provides you with a simple and quick method for testing your samples for proteolysis. Simply incubate your sample in the reagent provided and obtain results. The screening..
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Gene/Protein:
Rapidly, mix 1µl protein sample with the supplied Dilution Buffer and apply 1µl of the solution to the test strip by point-of-contact capillary action. Under the assay's specific buffer conditions the protein enters into the matrix of the test strip, binds and saturates as protein di..
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Protocols.io provides an interactive version of this protocol where you can discover and share optimizations with the research community.      ProteSEEKER™ identifies specific types of proteases with a panel of twelve protease inhibitors and a sensitive colorimetr..
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Protn-Latex™ is a simple and reliable method for estimation of protein contamination in latex. Protein estimation can be performed either on the production site or in any laboratory with a minimum of skill or instrumentation. The test detects as low as 25-40µg protein /gm latex (or 25-50..
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Recombinant human caspases 1-3 and 6-10 are supplied lyophilized in quantities of 25 units.   One unit of the specific recombinant caspase is the enzyme activity that cleaves 1nmol of a specific caspase substrate per hour at 37ºC in a reaction solution containing 50mM HEPES (pH7.2), 50mM..
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Gene/Protein:
A novel protein assay that is suitable for single sample or high throughput protein estimation. The SPN™ and SPN™-htp protein assays are rapid assays that are suitable for as little as 0.5µg protein and are resistant to interference from common laboratory agents. The assays are exc..
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