Bioassays
Our Bioassays cover a wide range of proteomic and cell biology fields. Our CytoScan™ products offer a range of
non-radioactive bioassays for the study of chemical and cell-mediated cytotoxicity, viability, cell death or cell proliferation. Included are cell proliferation and cell toxicity assays which feature lactate dehydrogenase (LDH) assays, WST-1 assays and sulforhodamine B (SRB) assays. Our apoptotic line features caspase assays and a wide selection of inhibitors.
We offer continuous enzyme coupled assays for methylation that can continuously monitor SAM-dependent methyltransferases without the use of radioactive labels or endpoint measurements.
We have a phosphatase assay for the quantification of phosphatases and three phosphatase inhibitor cocktails to inhibit phosphatases and for phosphatase studies.
Sensitive protease screening is needed to study protease activity present in the sample of interest. The Protease Screening Kit provides you with a simple and quick method for testing your samples for proteolysis.
Simply incubate your sample in the reagent provided and obtain results. The screening..
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Rapidly, mix 1µl protein sample with the supplied Dilution Buffer and apply 1µl of the solution to the test strip by point-of-contact capillary action. Under the assay's specific buffer conditions the protein enters into the matrix of the test strip, binds and saturates as protein di..
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Protocols.io provides an interactive version of this protocol where you can discover and share optimizations with the research community.
ProteSEEKER™ identifies specific types of proteases with a panel of twelve protease inhibitors and a sensitive colorimetr..
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Protn-Latex™ is a simple and reliable method for estimation of protein contamination in latex. Protein estimation can be performed either on the production site or in any laboratory with a minimum of skill or instrumentation. The test detects as low as 25-40µg protein /gm latex (or 25-50..
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Recombinant human caspases 1-3 and 6-10 are supplied lyophilized in quantities of 25 units. One unit of the specific recombinant caspase is the enzyme activity that cleaves 1nmol of a specific caspase substrate per hour at 37ºC in a reaction solution containing 50mM HEPES (pH7.2), 50mM..
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Methylation of key biological molecules and proteins plays important roles in numerous biological systems, including signal transduction, biosynthesis, protein repair, gene silencing and chromatin regulation (1). The S-adenosylmethionine (SAM) dependent methyltransferases use SAM, the..
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Trypan Blue 0.4% Solution is commonly used as a cell stain to measure cell viability using dye exclusion method. The dye exclusion method is based on the principle that cell impermeable dyes like Trypan blue will stain only dead cells where as viable cells will not be stained.
Trypan Blue staining ..
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β-Galactosidase Assay (CPRG) provides an easy, rapid, and highly sensitive method for determining the β-galactosidase activity in the lysates of cells transfected with a β-galactosidase expression construct. The β-Galactosidase Assay (CPRG) assay uses chlorophenol red-&beta..
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Protocols.io provides an interactive version of this protocol where you can discover and share optimizations with the research community.
Protein assays are routinely used in many research fields to estimate proteins in a vast array of buffers and conditions. A major prob..
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A reliable colorimetric assay that measures the stable, cytosolic, lactate dehydrogenase (LDH) enzyme, which is released from damaged cells and is a biomarker for cellular cytotoxicity and cytolysis. The released LDH is measured with a coupled enzymatic reaction that results in the conversion of a t..
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(9-fluoro-11β,17,21-trihydroxy-16a-methylpregna-1,4-diene-3,20-dione)
A potent and stable glucocorticoid steroid hormone that is often used as an anti-inflammatory and anti-rheumatic drug.
The mechanism of action is not fully understood, however it is thought that it may inhibit..
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Malachite Green Phosphate Assay is a simple, rapid, colorimetric kit for estimation of free phosphate in the solution. The assay is based on the principle of quantifying the green color complex formed between malachite green, molybdate and free orthophosphate. The complex formation is rapid and can ..
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