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Bioassays

Download the Bioassay Handbook & Selection Guide for Complete Product Listing

 

Our Bioassays cover a wide range of proteomic and cell biology fields. Our CytoScan products offer a range of
non-radioactive bioassays for the study of chemical and cell-mediated cytotoxicity, viability, cell death or cell proliferation. Included are cell proliferation and cell toxicity assays which feature lactate dehydrogenase (LDH) assays, WST-1 assays and  sulforhodamine B (SRB) assays. Our apoptotic line features caspase assays and a wide selection of inhibitors.

 
An array of highly sensitive protein quantification assays are available including NI(Non-Interfering) protein assay, a colorimetric protein assay that overcomes interference of common laboratory agents, and our CB-X  assay which is designed to be compatible with all commonly used buffers and conditions.

 

We offer continuous enzyme coupled assays for methylation that can continuously monitor SAM-dependent methyltransferases without the use of radioactive labels or endpoint measurements.

 

We have a phosphatase assay for the quantification of phosphatases and three phosphatase inhibitor cocktails to inhibit phosphatases and for phosphatase studies.

 
PEROXsay Peroxide Assays and a variety of enzymes are also available.
(4’-demethyl-epipodophyllotoxin 9-[4,6-O-(R)-ethylidene-beta-D-glucopyranoside], 4’ -(dihydrogen phosphate))     An inhibitor of topoisomerase II.     Supplied as 50μl 100mM solution.  ..
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The Fluorescent β-Galactosidase Assay (MUG) is a highly sensitive, fluorescent assay for determining the β-galactosidase activity in the lysates of cells transfected with a β-galactosidase expression construct   The study of the lac operon has played an important role in unders..
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The Fluoro™ Protease Assay Kit is designed for the quantitative determination of proteases present in a protein sample. The assay uses fluorescein isothiocyanate (FITC)-labeled casein as a general protease substrate. The fluorescein label on the FITC-casein is highly quenched. When the proteas..
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Glucose is the primary source of energy in most organisms from bacteria to humans. Glucose levels are tightly regulated in humans through homeostasis. Glucose homeostasis is of vital importance and insulin regulates the concentration of glucose in the blood with normal levels falling within 3.5..
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Reduced glutathione (L-γ-glutamyl-L-cysteinylglycine), a tripeptide, serves as a key antioxidant in animal, plant, fungi and bacteria by providing free thiol. Glutathione exist in reduced (GSH) and oxidized (GSSG; gluthathione disulphide) forms in cells and tissues, and the concentration of gl..
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Glutathione S-Transferase Assay [Colorimetric] is a type of ROS assay which is designed to measure total GST activity of crude cell or tissue extract and plasma. It is based on 1-chloro-2, 4-dinitrobenzene (CDNB) conjugation to the thiol groups of glutathione which increases absorbance at 340 nm. CD..
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Firefly Luciferase Assays are one of the widely used reporter assays to detect and measure gene expression, function and regulation.  G-Biosciences Lumino™ Firefly Luciferase Assay is designed for rapid and high sensitive detection or quantification of firefly luciferase reporter enzyme ..
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Lumino™ Firefly Luciferase Glow Assay is designed for high throughput screening or quantification of luciferase expression in mammalian cells which is mainly performed by batch processing of 96- and 384- well plates. Lumino™ Firefly Luciferase Glow Assay is homogenous, high-sensitive, g..
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The Phosphatase Assay kit is designed to measure the activity of phosphatases in biological samples and to screen for agonists and inhibitors of phosphatases.   The Phosphatase Assay kit uses para-nitrophenyl phosphate (pNPP), a chromogenic substrate for most phosphatases, including alkaline ..
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PhosphoQuant™ is specifically designed for quick and reliable determination of whether a purified protein is phosphorylated and the extent of phosphorylation. The assay is based on the alkaline hydrolysis of phosphates from seryl and threonyl residues in phosphoproteins and the subsequent quantifica..
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The Protease Assay Kit is designed for the quantitative determination of proteases present in a protein sample, using a dye-labeled protein substrate. The proteases present in the sample of interest will digest the protein substrate and release dye labeled peptides. The absorbance of the dye-labele..
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RESORUFIN-CASEIN PROTEASE SUBSTRATE A colorimetric substrate that when treated with proteases releases resorufin that has an absorbance of 570nm. Supplied lyophilized.   FITC-CASEIN PROTEASE SUBSTRATE A fluorescent (fluorescein isothiocyanate (FITC)) substrate that when treated with p..
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