Bioassays
Our Bioassays cover a wide range of proteomic and cell biology fields. Our CytoScan™ products offer a range of
non-radioactive bioassays for the study of chemical and cell-mediated cytotoxicity, viability, cell death or cell proliferation. Included are cell proliferation and cell toxicity assays which feature lactate dehydrogenase (LDH) assays, WST-1 assays and sulforhodamine B (SRB) assays. Our apoptotic line features caspase assays and a wide selection of inhibitors.
We offer continuous enzyme coupled assays for methylation that can continuously monitor SAM-dependent methyltransferases without the use of radioactive labels or endpoint measurements.
We have a phosphatase assay for the quantification of phosphatases and three phosphatase inhibitor cocktails to inhibit phosphatases and for phosphatase studies.
Gene/Protein:
CytoScan™-SRB Cytotoxicity Assay is an accurate and reproducible assay based upon the quantitative staining of cellular proteins by sulforhodamine B (SRB).
This assay has been used for high-throughput drug screening at the National Cancer Institute (NCI) (1). Sulforhodamine B ..
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Gene/Protein:
CytoScan™ WST-1 Cell Proliferation Assay is a sensitive and accurate assay for cell proliferation and cytotoxicity. The assay is highly convenient as it is performed in a single cell tissue culture well and requires no washing, harvesting or solubilization of cells.
Adheren..
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Gene/Protein:
The CytoScan™-fluoro is based on the quantification of cellular lactate dehydrogenase (LDH) released when cells are damaged or under stress. Lactate dehydrogenase released into the culture medium is measured with a diaphorase coupled enzymatic assay that results in the conversion of a non-fluo..
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Gene/Protein:
Dimethylsulfoxide (DMSO) is a very polar organic compound which used for solubilizing organic and inorganic compounds. In addition, DMSO is used in cell culture as a cryoprotectant added to cell culture medium, to prevent formation of ice crystals during freezing of cells. DMSO compound c..
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((8S,10S)-10-(4-amino-5-hydroxy-6-methyl-tetrahydro-2H-pyran-2-yloxy)-6,8,11-trihydroxy-8-(2-hydroxyacetyl)-1-methoxy-7,8,9,10-tetrahydrotetracene-5,12-dione)
Doxorubicin (or hydroxyldaunorubicin) is an anthracycline antibiotic that intercalates DNA, inhibiting the unwinding of DNA b..
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(4’-demethyl-epipodophyllotoxin 9-[4,6-O-(R)-ethylidene-beta-D-glucopyranoside], 4’ -(dihydrogen phosphate)) An inhibitor of topoisomerase II. Supplied as 50μl 100mM solution. ..
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The Fluorescent β-Galactosidase Assay (MUG) is a highly sensitive, fluorescent assay for determining the β-galactosidase activity in the lysates of cells transfected with a β-galactosidase expression construct
The study of the lac operon has played an important role in unders..
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Gene/Protein:
The Fluoro™ Protease Assay Kit is designed for the quantitative determination of proteases present in a protein sample. The assay uses fluorescein isothiocyanate (FITC)-labeled casein as a general protease substrate. The fluorescein label on the FITC-casein is highly quenched. When the proteas..
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Gene/Protein:
Reduced glutathione (L-γ-glutamyl-L-cysteinylglycine), a tripeptide, serves as a key antioxidant in animal, plant, fungi and bacteria by providing free thiol. Glutathione exist in reduced (GSH) and oxidized (GSSG; gluthathione disulphide) forms in cells and tissues, and the concentration of gl..
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Firefly Luciferase Assays are one of the widely used reporter assays to detect and measure gene expression, function and regulation.
G-Biosciences Lumino™ Firefly Luciferase Assay is designed for rapid and high sensitive detection or quantification of firefly luciferase reporter enzyme ..
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Lumino™ Firefly Luciferase Glow Assay is designed for high throughput screening or quantification of luciferase expression in mammalian cells which is mainly performed by batch processing of 96- and 384- well plates.
Lumino™ Firefly Luciferase Glow Assay is homogenous, high-sensitive, g..
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Gene/Protein:
The Phosphatase Assay kit is designed to measure the activity of phosphatases in biological samples and to screen for agonists and inhibitors of phosphatases.
The Phosphatase Assay kit uses para-nitrophenyl phosphate (pNPP), a chromogenic substrate for most phosphatases, including alkal..
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