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Bioassays

Download the Bioassay Handbook & Selection Guide for Complete Product Listing

 

Our Bioassays cover a wide range of proteomic and cell biology fields. Our CytoScan products offer a range of
non-radioactive bioassays for the study of chemical and cell-mediated cytotoxicity, viability, cell death or cell proliferation. Included are cell proliferation and cell toxicity assays which feature lactate dehydrogenase (LDH) assays, WST-1 assays and  sulforhodamine B (SRB) assays. Our apoptotic line features caspase assays and a wide selection of inhibitors.

 
An array of highly sensitive protein quantification assays are available including NI(Non-Interfering) protein assay, a colorimetric protein assay that overcomes interference of common laboratory agents, and our CB-X  assay which is designed to be compatible with all commonly used buffers and conditions.

 

We offer continuous enzyme coupled assays for methylation that can continuously monitor SAM-dependent methyltransferases without the use of radioactive labels or endpoint measurements.

 

We have a phosphatase assay for the quantification of phosphatases and three phosphatase inhibitor cocktails to inhibit phosphatases and for phosphatase studies.

 
PEROXsay Peroxide Assays and a variety of enzymes are also available.
Gene/Protein:
An improved Coomassie Dye based protein assay based on the Bradford Protein Assay (1). This assay is suitable for the simple and rapid estimation of protein concentration. This assay is based on a single Coomassie dye based reagent. The binding of protein to the dye results in a change of color from..
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Free radicals are highly reactive species that are naturally formed during cell metabolism in the mitochondria, secretion by immune cells during inflammatory processes or taken up as environmental pollutants. CUPRAC (CUPric Reducing Antioxidant Capacity) Assay is a new method, which enables the To..
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Gene/Protein:
(4-{(2R)-2-[(1S,3S,5S)-3,5-dimethyl-2-oxocyclohexyl]-2-hydroxyethyl}piperidine-2,6-dione)     An inhibitor of protein synthesis by interfering with the translocation step (movement of two tRNA molecules and mRNA on ribosome) in protein synthesis, thus blocking translational elongati..
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Gene/Protein:
CytoScan™-SRB Cytotoxicity Assay is an accurate and reproducible assay based upon the quantitative staining of cellular proteins by sulforhodamine B (SRB).   This assay has been used for high-throughput drug screening at the National Cancer Institute (NCI) (1). Sulforhodamine B ..
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CytoScan™ WST-1 Cell Proliferation Assay is a sensitive and accurate assay for cell proliferation and cytotoxicity. The assay is highly convenient as it is performed in a single cell tissue culture well and requires no washing, harvesting or solubilization of cells.     Adherent or..
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The CytoScan™-fluoro is based on the quantification of cellular lactate dehydrogenase (LDH) released when cells are damaged or under stress. Lactate dehydrogenase released into the culture medium is measured with a diaphorase coupled enzymatic assay that results in the conversion of a non-fluo..
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Dimethylsulfoxide (DMSO) is a very polar organic compound which used for solubilizing organic and inorganic compounds. In addition, DMSO is  used in cell culture as a cryoprotectant added to cell culture medium, to prevent formation of ice crystals during freezing of cells. DMSO can be use..
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((8S,10S)-10-(4-amino-5-hydroxy-6-methyl-tetrahydro-2H-pyran-2-yloxy)-6,8,11-trihydroxy-8-(2-hydroxyacetyl)-1-methoxy-7,8,9,10-tetrahydrotetracene-5,12-dione)     Doxorubicin (or hydroxyldaunorubicin) is an anthracycline antibiotic that intercalates DNA, inhibiting the unwinding of DNA b..
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(4’-demethyl-epipodophyllotoxin 9-[4,6-O-(R)-ethylidene-beta-D-glucopyranoside], 4’ -(dihydrogen phosphate))     An inhibitor of topoisomerase II.     Supplied as 50μl 100mM solution.  ..
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The Fluorescent β-Galactosidase Assay (MUG) is a highly sensitive, fluorescent assay for determining the β-galactosidase activity in the lysates of cells transfected with a β-galactosidase expression construct   The study of the lac operon has played an important role in unders..
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Gene/Protein:
The Fluoro™ Protease Assay Kit is designed for the quantitative determination of proteases present in a protein sample. The assay uses fluorescein isothiocyanate (FITC)-labeled casein as a general protease substrate. The fluorescein label on the FITC-casein is highly quenched. When the proteas..
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Gene/Protein:
Reduced glutathione (L-γ-glutamyl-L-cysteinylglycine), a tripeptide, serves as a key antioxidant in animal, plant, fungi and bacteria by providing free thiol. Glutathione exist in reduced (GSH) and oxidized (GSSG; gluthathione disulphide) forms in cells and tissues, and the concentration of gl..
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