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Mitochondrial Assays

Download the Bioassay Handbook & Selection Guide for Complete Product Listing

 

Mitochondria are referred to as “power-houses of cell” as they generate the majority of cellular ATP via oxidative phosphorylation. Furthermore, ß-oxidation of short-, medium and long- chain fatty acids is carried in mitochondria and mitochondria are involved in ROS generation and apoptosis. Mitochondria also play a key role in intermediary metabolism. 
Mitochondrial dysfunction has been linked to variety of market therapeutics and also to several pathological diseases. Therefore studying or checking mitochondrial health is paramount to cell health. 

FCCP [Carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone] is a potent uncoupler of oxidative phosphorylation in mitochondria. It is used as a positive control for mitochondrial membrane depolarization. FCCP disrupts the synthesis of ATP by trnasporting protons across mitochondrial inner membrane..
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Gene/Protein:
JC-1 Mitochondrial Membrane Potential Assay kits comprise lipophilic cationic fluorescent dye; 5,5,6,6’-tetrachloro-1,1’,3,3’-tetraethylbenzimi-dazoylcarbocyanine iodide (JC-1).  In healthy cells, JC-1 enters the energized mitochondria and form aggregates which change the fluo..
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Gene/Protein:
Mitochondrial membrane potential is one of the key parameters of mitochondrial function and serves as an indicator of cell health. JC-10 Mitochondrial Membrane Potential Assay is based upon JC-10 dye, which is a superior alternative to JC-1 dye due to its increased solubility. JC-10 sho..
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TMRE Mitochondrial Membrane Potential Assay quantifies changes in mitochondrial membrane potential of live cells via microplate fluorometry, fluorescence microscopy and flow cytometry. Tetramethylrhodamine, ethyl ester (TMRE) is a cell permeable, positively charged red-orange dye that accumulates i..
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