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Labeling & Conjugation

Download the Protein Labeling & Conjugation Handbook

 

G-Biosciences offer a vast array of products for protein labeling and conjugations. Both fluorescent and biotin labeling kits are available as well as products for agarose conjugation and accessories.

 

Fluorescent dyes have become a common tool in laboratories for the visualization and studying of proteins, whether they are attached directly to the protein of interest or to antibodies specific for the protein of interest. 

G-Biosciences researchers have developed two kits for the rapid and efficient labeling of proteins, including antibodies, with a fluorescent green (FITC) or red ((5/6) TAMRA-SE) dye.  Each kit is supplied with five of our single-use OneQuant™ fluorescent reagents that protect the dyes from exposure to light and minimize hands-on time.  The following evaluates labeling of two different proteins with fluorescent dyes.

 

HOOK-Biotin kits come with all the necessary reagents and equipment for optimization of reaction conditions, efficient labeling, removal of unbound biotin and quantification of biotin labeling.  In addition to highly efficient labeling, the HOOK-Biotin kits offer the advantage of being supplied with micro dialysis units and a specific Optimizer Buffer.  These greatly simplify the labeling process and ensure high levels of biotin labeling.

Biotin, a 244Da vitamin (Vitamin H) molecule, exhibits an extraordinary binding affinity for avidin (Ka=10¹âµM-¹) and streptavidin (Ka=10¹âµM-¹). Biotin and (strept)avidin interaction is rapid. Once the bond is established, it can survive up to 3M guanid..
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Tube-O-Reactor™ is a system that allows all the key steps of cross linking, coupling and modification of proteins and/or nucleic acids to be performed in a single tube. This minimizes the risk of sample loss, of experimental time and of hands-on phases.   Most of the above reactions inv..
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Model: Swift™ Dye Labeling Kit (With Alexa Fluor®594)
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$295.00
Model: Swift™ Dye Labeling Kit (With Alexa Fluor®594)
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$395.00
Model: Swift™ Dye Labeling Kit (With Alexa Fluor®647)
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$395.00
Model: Sulfhydration Kit With Traut's Reagent
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$260.00
The amine reactive HOOK™ Activated Agarose is 6% agarose that has been activated to generate reactive aldehyde groups. The aldehyde groups of the agarose react spontaneously with primary amines, located at the N-terminus of proteins or in lysine residues, to form intermediate Schiff Base compl..
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The HOOK™ HRP PLUS labeling kit is a high efficiency horseradish peroxidase (HRP) enzyme labeling kit for tagging the enzyme with proteins.  The HRP is activated by treating it with metaperiodate which produces the aldehyde groups in carbohydrate moiety of HRP. This activated HRP (HRP PLU..
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The HOOK™ HRP SULFO labeling kit is an efficient enzyme labeling kit for tagging proteins with horseradish peroxidase (HRP).  The kit contains activated HRP that couples to peptides, proteins and ligands that have free sulfhydryl groups. The HRP has been maleimide-activated using Sulfo-SM..
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Alkaline Phosphatase is a hydrolase enzyme, which is frequently conjugated to antibodies for its use in immunoassays. p-Nitrophenol phosphate (pNPP) is the most commonly used substrate for Alkaline Phosphatase. Alkaline Phosphatase converts pNPP to p-nitrophenol phosphate (pNP), a colored product, w..
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Phycobiliproteins are fluorescent proteins obtained from cyanobacteria and eukaryotic algae. The fluorescence of these proteins is very high, when compared to chemical fluorescent probes, such as fluorescein and rhodamine. Allophycocyanin (APC) is a fluorescent protein isolated from cyanobacteria al..
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Avidin, an egg-white protein is a tetrameric biotin binding protein. It is highly cationic glycoprotein with molecular weight of around 66kD. The protein contains four identical units, each of it binds to biotin with high specificity and affinity. The dissociation constant (KD) of avidin-biotin comp..
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