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Western Blotting

Download the Western Blotting Handbook for Troubleshooting Tips

 

Transfer systems, chemiluminescence and chromogenic detection systems, blocking and wash buffers, antibodies and enzyme labelling kits.

Based on Efficient™ Western Transfer Buffer, the High Molecular Weight Transfer buffer is designed to facilitate the transfer of notoriously difficult high molecular weight proteins (>70kDa) during Western blotting.   Supplied as 1 liter of a 5X concentrated solution...
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For user's convenience, we offer pre-cut transfer membranes and padding for Western blot transfer procedures. Pre-cut membranes are supplied sandwiched between blotting paper padding. Simply soak the membrane in transfer buffer and assemble with the gel in a transfer cassette. Nitrocellulose, (0..
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A 10X Concentrated solution of Phosphate Buffered Saline with Tween® 20. The 10X Concentration is 80mM Na2HPO4, 1.5M NaCl, 20mM KH2PO4, 30mM KCl, 0.5% Tween® 20, pH 7.4.   PBST is commonly used as a wash solution for Western blot membranes and microtiter plate wells in ELISA ..
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picoLUCENT™ PLUS-AP kit is based on our ultra sensitive Luminol substrate that produces chemiluminescence upon reaction with horseradish peroxidase. The chemiluminescence light emission can be recorded by a short exposure to autoradiography films.    picoLUCENT™ PLUS-AP is al..
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Protocols.io provides an interactive version of this protocol where you can discover and share optimizations with the research community.    picoLUCENT™ PLUS-HRP kit is based on our ultra sensitive Luminol substrate that produces chemiluminescence upon reaction with horseradish per..
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Protein-Free Blocking Buffer does not contain protein; it is a proprietary formation of non-protein agents that eliminates non-specific binding sites in ELISA, blotting, immunohistochemistry and other applications.  The absence of protein from the Protein-Free Blocking Buffer eliminates problem..
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 Our PVDF (polyvinylidene) membranes are pre-cut membranes for Western transfers, or Western blotting.  The PVDF membranes bind biomolecules, including proteins, through hydrophobic interactions The presence of the 0.45µm pore sizes increases the surface binding area. Typical thickn..
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We offer a range of secondary antibodies conjugated to either alkaline phosphatase (AP) or horseradish peroxidase (HRP). The antibodies are isolated from antisera by immuno-affinity chromatography using antigen coupled to sepharose beads or other methods. They are supplied lyophilized, from a buffer..
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Superior™ Blocking Buffer contains a proprietary antiginically non-determinant protein for blocking non-specific sites during ELISA, membrane blotting, immunohistochemistry and other applications.    Superior™ Blocking Buffer is ideal for a high signal to background ratio in ..
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Swift™ Membrane Stain is a unique, proprietary (patents pending), rapid, reversible, ready-to-use membrane stain for proteins on nitrocellulose or PVDF membranes. Swift™ Membrane Stain stains proteins faster and with 500 times more sensitivity than the routinely used Ponceau-S stain and ..
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Swift Film Cleaner™ allows researchers to clean film that has been overexposed or has a high background or speckling without having to repeat experiments.  The Swift Film Cleaner™ is suitable for all experiments that use film to develop the result, including gel shift assays, Wester..
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Protocols.io provides an interactive version of this protocol where you can discover and share optimizations with the research community.    Western blot analysis of proteins is a routine and commonly used technique in research laboratories, with 3 major drawbacks. The first is the amoun..
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