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Cross-Linking & Modification

Download the Crosslinkers Handbook & Selection Guide            Download the Protein Labeling & Conjugation Handbook

 

 Cross-linking agents contain at least two reactive groups that are reactive towards numerous groups, including sulfhydryls, amines and carbohydrates, and create chemical covalent bonds between two or more molecules.  functional groups that can be targeted with cross-linking agents are primary amines, carboxyls, sulfhydryls,  carbohydrates and carboxylic acids. Protein molecules have many of these functional groups and therefore proteins and peptides can be readily conjugated using cross-linking agents. Cross-linking agents are used to study protein structure and function, to anchor proteins to solid  supports, preparation of immunogens, immunotoxins, and other conjugated protein reagents. G-Biosciences offers a variety of Double Do™ cross-linking agents, reaction specific buffers, and accessories for performing and facilitating crosslinking applications. These accessory tools simplify cross-linking reactions and will enable researchers to achieve highly efficient cross-linking reactions with minimal effort in reaction optimization, set-up, and procedure development

 
We offer a variety of amino acid side chain modifiers to block side chain interactions, change the charge or modify them to be more favorable for other reactions. Many denaturants, or chaotropes, are available and can disrupt water interactions resulting in the solubilization of hydrophobic proteins and peptides. Chaotropes act on proteins to unfold them and alter their three-dimensional structure. A wide selection of reagents and accessories are also available.
N-Sulfosuccinimidyl(4-iodoacetyl)aminobenzoate   Features Molecular Weight: 504.19 Spacer Arm (Å): 10.6 Reactive Toward: Primary Amine + Sulfhydryl Membrane Permeable: NO Water Soluble: YES Cleavable/ Reversible: NO    ..
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Sulfosuccinimidyl 4-(N-maleimidomethyl) cyclohexane-1-carboxylate   Ideal for enzyme-antibody crosslinking   OneQuant™ Sulfo SMCC is also available: Single use vials to minimize waste. No weighing required.   Features Synonym: Water Soluble SMCC CAS Number: 9292..
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Sulfo succinimidyl 4-(ρ-maleimidophenyl) butyrate   Features Molecular Weight: 458.38 Spacer Arm (Å): 14.5 Reactive Toward: Primary Amine + Sulfhydryl  Membrane Permeable: NO Water Soluble: YES Cleavable/ Reversible: NO    ..
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Features Chemical Name: 2-Iminothiolane hydrochloride Molecular weight: 137.63 Mild conditions: pH 7-10, 25°C Soluble in water   Applications For the addition of sulfhydryls to primary amines For the preparation of disulfide bridges or generation of sulfhydryl ..
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Tube-O-Reactor™ is a system that allows all the key steps of cross linking, coupling and modification of proteins and/or nucleic acids to be performed in a single tube. This minimizes the risk of sample loss, of experimental time and of hands-on phases.   Most of the above reactions inv..
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Sulfo-SANPAH   N-Sulfosuccinimidyl-6-(4'-azido-2'-nitrophenylamino) hexanoate   Photoreactive Crosslinker   Sulfo-SANPAH is a water-soluble crosslinker with a long spacer-arm (18.2 angstrom) that contains an amine-reactive N-hydroxysuccinimide (NHS) ester an..
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TCEP is a water-soluble, odorless, non-toxic and stable protein reductant. This potent reducing agent is a popular alternative to ß-mercaptoethanol and DTT (dithiothreitol) use for most applications. The reduction potency of TCEP is twice as high as that of DTT, and TCEP is effective in reduci..
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A Chemically Modified, TPCK treated, Affinity Purified Trypsin. G-Biosciences' Mass Spectrometry Grade Trypsin is chemically methylated to yield an enzymatically active protein with maximum trypsin specificity and extreme resistance to autolysis. In addition, the modified trypsin is TPCK tr..
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High quality proteomic grade β-mercaptoethanol.   β-mercaptoethanol, a popular reducing agent, is offered in 5ml and 100ml bottles.   Features Synonym: β-Mercaptoethanol CAS#: 60-24-2 Molecular Formula: C2H6OS Molecular Weight: 78.13  ..
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