PCR Reagents

G-Biosciences offers a wide selection of affordable regular and high-fidelity DNA polymerases and deoxynucleotides (dNTPs) for performing PCR. These are high quality enzymes and dNTPs for successful and reproducible PCR. The following enzymes are offered in three different formats: I) Regular, II) Hot start and III) 2x Master Mix.

I) DNA Polymerases:

• Taq DNA Polymerase possesses a 5´→3´ polymerase activity and a 5´-flap endonuclease activity that can amplify DNA up to 5kb with an elongation velocity of 0.9-1.2kb/min at 70-75°C.  
• PFU DNA polymerase has superior thermostability and proofreading properties compared to Taq DNA polymerase. It can amplify DNA target up to 2kb. The elongation velocity is 0.2~0.4kb/min at 70~75°C. Also possesses 3' to 5' exonuclease proofreading activity that enables the polymerase to correct nucleotide-misincorporation errors. 
• Taq Plus DNA Polymerase is a mixture of Taq DNA Polymerase and Pfu, a proofreading DNA Polymerase, which allows for the amplification of long templates, up to 20kb, with high fidelity.
• High fidelity DNA Polymerase possesses 5´→3´ polymerase and 3´→5´ exonuclease (proofreading) activities. This Polymerase has the fidelity, sensitivity and processivity with an error rate ~2.8×10²-fold lower than Taq DNA polymerase, and significantly lower than other proofreading DNA polymerases. 

II) Hot start DNA Polymerases:

• Hot start Taq DNA Polymerase is chemically modified that possesses a 5´→3´ polymerase activity and a 5´-flap endonuclease activity. Hot start Taq DNA Polymerase reduces non-specific amplification during PCR setup and increases PCR specificity and sensitivity.
• Hot start high fidelity DNA Polymerase is chemically modified that possesses 5´→3´ polymerase and 3´→5´ exonuclease (proofreading) activities.  It has the fidelity, sensitivity and processivity with an error rate ~2.8×10²-fold lower than Taq DNA polymerase. It reduces non-specific amplification during PCR setup and increases PCR specificity, sensitivity and processivity.

 III) DNA Polymerases 2X Master Mix:

• Taq DNA Polymerase 2X master mix is ready to use premix which contains Taq DNA Polymerase, dNTPs and optimized buffer with stabilizers. Taq DNA Polymerase possesses a 5´→3´ polymerase activity and a 5´-flap endonuclease activity.
• Hot start Taq DNA Polymerase 2x master mix is ready to use premix which contains hot start Taq DNA Polymerase, dNTPs and optimized buffer with stabilizers. Hot start Taq DNA Polymerase reduces non-specific amplification during PCR setup and increases PCR specificity and sensitivity.
• Pfu DNA Polymerase 2X master mix is a premixed, ready-to-use solution containing Pfu DNA Polymerase, dNTPs, MgSO₄ and Reaction Buffer at optimal concentrations for efficient amplification of DNA templates by PCR. Pfu Mix contributes to highly reproducible PCR by reducing the risk of pipetting errors, miscalculation and contamination.
• Taq Plus DNA Polymeras 2X master mix is a premixed, ready-to-use solution containing Taq Plus DNA Polymerase, dNTPs, Mg2+ and Reaction Buffer at optimal concentrations for efficient amplification of DNA templates by PCR. To prepare the final PCR, only primers and template DNA are added.
• High fidelity DNA Polymerase 2x master mix is ready to use premix which contains high fidelity DNA Polymerase, dNTPs and optimized buffer with stabilizers. The high-fidelity DNA Polymerase is used for high fidelity PCR, cloning, and long and high GC content template DNA amplification.
• Hot start high fidelity DNA Polymerase 2x master mix is ready to use premix which contains chemically modified hot start high fidelity DNA Polymerase, dNTPs and optimized buffer with stabilizers. Hot start high fidelity DNA Polymerase reduces non-specific amplification during PCR setup and increases PCR specificity and sensitivity. It is used for high fidelity PCR, cloning, and long and high GC content template DNA amplification.

IV) Deoxynucleotides (dNTPs):

The dNTPs are supplied as

• an equimolar mixture of dATP, dCTP, dGTP and dTTP that are available at 10 and 25mM concentrations
• a set of individual dNTPS 
• individually at 100mM.

Related Products:

In addition to the DNA polymerases and dNTPS, several kits are offered for the 

• Extraction of Genomic DNA  from a large selection of tissues and specimens
• Plasmid Purification & Colony Screening
• PCR clean up following the PCR.