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Protein Electrophoresis

Download the Protein Electrophoresis Handbook for Full Product List


Protein gel electrophoresis is a technique used to separate and visualize proteins based on their size and charge. 1D and 2D gel protein electrophoresis is an invaluable tool for the modern biologist and G-Biosciences provide a full range of products to help achieve the results you need.

A wide variety of sample preparation reagents for 1D and 2D protein gel electrophoresis are available from G-Biosciences. PAGE-Perfect™ is a simple method for removing contaminants and improving resolution for your publication quality gels. Immobilized Reductant is an excellent way to remove reducing agents from your protein preps and improve your downstream results. To remove salts, detergents and other buffering agents we provide PAGE-Optimizer™, a spin column for the preparation of samples for SDS-PAGE.

Two reliable protein standards are available from G-Biosciences.  PAGEmark™ Blue Plus is a 12 protein prestained  ladder with a molecular weight range from 10-240kDa (in Tris-Glycine buffer) and 9-235kDa (in MOPS or MEPS buffer).  PAGEmark™ Tricolor Plus is a three color, 12 protein standard that has a molecular weight range of 5-245kDa  (in Tris-Glycine buffer) or 3.5-235kDa (in MOPS or MEPS buffer). 

Also available are a wide range of gel stains and destains to insure the best visualization of your gel protein electrophoresis. We offer Coomasie Brilliant BlueRAPIDstain™Reversible Copper Stain™, and several other stains optimized for every downstream application.

Our partners for benchtop equipment, BTLabs provide gel electrophoresis boxes, power supplies, dry bath incubators and wide range of buffers and chemicals for your electrophoresis needs.

Our 2D protein gel electrophoresis products are optimized for isoelectric focusing (IEF) and 2D electrophoresis. 2D detergents such as 2-D Detergent™ Triton®X-100 and 2-D Detergent™ Nonidet® P-40 Substitute contain reduced levels of sulfhydryl oxidizing agents, peroxides, salts and carbonyl compounds. Reducing these agents makes the protein less susceptible to oxidation and the formation of Schiff’s bases which interfere with a protein’s native structure.

For more information on protein gel electrophoresis refer to the Proteinman’s Blog and download our free Protein Electrophoresis Handbook.


PAGE-Optimizer™ is a unique, spin column for the preparation of samples for SDS-PAGE.  The columns contain a proprietary separation matrix that removes contaminants such as salts, detergents, cellular agents and other common buffering agents.     PAGE-Optimizer™ helps el..
Remove Electrophoretic Contaminants for Improved Protein Resolution and Publication Quality Gels; Ideal for Difficult or Dilute Samples   Many lysis buffers and reagents used in sample preparation are incompatible with routinely used electrophoretic analysis. The presence of contaminants, or ..
PAGEmark™ Blue PLUS Protein Markers A blue protein standard with 12 prestained proteins covering a wide range of molecular weights: 10-240kDa in Tris-Glycine buffer and 9-235kDa in Bis-Tris (MOPS) buffer or Bis-Tris (MES) buffer.   Proteins are covalently coupled with a..
Protein samples loaded on iso-electric focusing (IEF) gels should ideally have low conductivity and be free from agents known to interfere with net protein charge. Protein samples containing interfering agents, including ionic detergents, metal ions, salts, lipids, charged polysaccharides, peptides,..
A water soluble, odorless, non-toxic and stable protein reductant. Protein-S-S-Reductant™ uses TCEP (Tris [2-carboxyethyl] phosphine), a popular alternative to ß-mercaptoethanol and DTT (dithiothreitol). TCEP improves stability, increases effectiveness, and reduces proteins over a wider ..
Protein preparations specifically prepared for standardizing electrophoresis methods and protocols.   Substantially free from non-protein agents such as nucleic acids, detergents, salts, lipids and other common laboratory agents. The protein preparation has low conductivity (< 50µS..
A reagent kit for detection and estimation of SDS in a sample. Mix the test sample in the extraction buffer reagents provided with the kit. If SDS is present in the sample, a blue color is extracted that can be quantitatively measured.   The working range of the assay is 0.02-0.2% SDS...
The fixing of proteins in polyacrylamide gels prevents the diffusion of the proteins, thus keeping protein bands sharp and correctly resolved, and washes away running buffer components that may interfere with other downstream applications, such as staining.  A major interfering agent is the SDS..
The TPE™ kit is a universal lysis system designed for the solubilization and extraction of total proteins from cells and tissues for SDS-PAGE Analysis.  The kit is suitable for animal, plant, yeast, bacteria, and other biological samples.   The TPE™ kit provides a two compo..
Proteomic Grade Water suitable for 2D electrophoresis and mass spectrometry analysis. Removes worries of protien and dust contamination and improves quality and reproducibility of 2D electrophoresis and mass spectrometry results.   Molecular Grade, Endotoxin-Free a..
TCEP is a water-soluble, odorless, non-toxic and stable protein reductant. This potent reducing agent is a popular alternative to ß-mercaptoethanol and DTT (dithiothreitol) use for most applications. The reduction potency of TCEP is twice as high as that of DTT, and TCEP is effective in reduci..
High quality proteomic grade β-mercaptoethanol.   β-mercaptoethanol, a popular reducing agent, is offered in 5ml and 100ml bottles.   Features Synonym: β-Mercaptoethanol CAS#: 60-24-2 Molecular Formula: C2H6OS Molecular Weight: 78.13   Applications ..
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