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Protein Electrophoresis

Download the Protein Electrophoresis Handbook for Full Product List

 

A major tool in the analysis of a species' proteome is the ability to separate and visualize the proteins by electrophoresis, 1D or 2D, and then the subsequent visualization of the proteins. Electrophoresis is a quick, simple and sensitive tool for separating proteins and nucleic acids by size and/or electrical charge. G-Biosciences offers a wide selection of electrophoresis kits, reagents and accessories. The G-Biosciences’ electrophoresis range extends from sample preparation for electrophoresis to running the protein samples by 1D and 2D electrophoresis.
 
G-Biosciences has also developed a wide selection of electrophoresis gel stains for visualizing proteins that vary in sensitivity, staining time and reversibility.
 
A wide selection of buffers and chemicals for gel casting and electrophoresis are also available.

A suitable protein solubilization buffer for 2D analysis must solubilize proteins effectively, without disturbing the native charge of the proteins. Urea based solubilization buffers solubilize proteins effectively, however can modify the native charge of the proteins, due to carbamylation. Ure..
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Features CAS #: 79-06-1 Chemical Formula: C3H5NO Molecular Weight: 71.08   ..
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Simply heat and use for sealing the IPG strips for SDS-PAGE analysis. The Agarose Sealing Solution is prepared in a proprietary buffer to minimize reoxidation of the competing thiol pairs as proteins enter into the second dimension gel. Improves resolution and prevents streaking of protein spots on ..
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1,4 Dithiothreitol (DTT) is a small molecule reducing agent, also known as Cleland’s reagent. G-Biosciences DTT is a high quality proteomic grade DTT and is supplied in various sizes, including a OneQuant™ version. The OneQuant™ format features 40 vials of 7.7mg DTT for conven..
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Add an appropriate volume of the supplied rehydration buffer to the dry, ready-to-use, urea based equilibration buffer.   Eliminates worries about urea induced protein carbamylation or waste of unused reagents, as fresh and ready-to-use reagents are prepared every time.   The Equilibra..
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For those who prefer DTT as a reducing agent, we offer ultra pure proteomic grade DTT for protein reduction. FOCUS™ DTT is supplied as 5 vials containing dry powder to maintain the activity of the reducing agent. Reconstitute in 0.8ml proteomic grade water for a final volume of 1ml at 2M ..
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The kit is supplied with a proprietary buffer necessary for efficient alkylation of thiols, while minimizing reoxidation of the competing thiol pairs in protein samples. Simply add an appropriate amount to reagent solutions for alkylation of protein thiol groups.  Features  Pro..
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A water soluble, odorless, non-toxic and stable TCEP [Tris (2-carboxyethyl) phosphine] reductant for protein reduction.   FOCUS™ Protein Reductant has improved stability and efficiency compared to DTT and reduces proteins over a wide range of pH, including lower acidic pH. FOCUS™ ..
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The FOCUS™ Reduction-Alkylation kit offers a simple two-step method for reduction and alkylation of protein samples for 2D gel analysis.   The disulfide bonds are reduced with a highly reactive and stable TCEP [Tris (2-carboxyethyl) phosphine] followed by blocking of the thiols by alkyl..
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Spray and Wipe; Specifically Formulated For Electrophoresis Gel Plates   Clean your electrophoresis plates with GELPLATE-clean™ and prevent poor electrophoretic band migration, band distortions, and poor image development. Simply spray and wipe clean your electrophoresis plates with GEL..
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A proteomic grade iodoacetamide in two different quantities. Iodoacetamide is supplied in 5gm bulk quantities or in the OneQuant™ format.   OneQuant™ Iodoacetamide OneQuant™ iodoacetamide are single aliquots of iodoacetamide that eliminate the need for weighing, preventing ..
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LDS Sample Loading Buffer [4X] is used to prepare protein samples for SDS polyacrylamide gel electrophoresis.   Features Alkaline pH (pH 8.4) for optimal conditions for denaturation and reduction of disulfide bonds 4X concentrated allows for larger sample loading It has Lithium Dodecyl S..
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