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Affinity Purification Resins

Download the Protein Purification Handbook for a full list of related productsDownload the Purification Resins Handbook for Complete Product List

 

For the separation & purification of affinity tagged proteins & antibodies
 
The separation and purification of proteins has been a major challenge for researchers in the early days of proteomic research. The advances in affinity chromatography have enabled researchers to purify large quantities of highly pure proteins for a multitude of analysis techniques, including crystallography, protein:protein studies and in-vitro assays.
Affinity chromatography works by binding a protein, via a reversible interaction, to a specific ligand that is prebound to a solid chromatographic support. The protein(s) are first bound to the column in a buffer that supplies conditions optimal for binding. Unbound, non-specific material is washed away and the protein(s) of interest are then eluted by changing the buffering conditions to induce desorption from the solid support.
We supply two main groups of affinity resins. The first group is for the separation and purification of affinity tagged proteins and the second group is for the binding of immunoglobulin molecules.
A common practice in today’s research is the use of molecular biology to clone our protein(s) of interest into a vector that adds a specific tag to the protein. The most versatile and common tags used are glutathione S-transferase (GST), a 6x histidine motif (His-tag), and the calmodulin binding peptide (CBP).
For the generation of peptide and protein affinity columns, for the purification of antibodies and for the discovery of important interacting proteins and cofactors. G-Biosciences offers activated agarose for the coupling of peptides and proteins to agarose through their primary amines.   The..
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An important tool in research is the generation of peptide and protein affinity columns for the purification of antibodies and for the discovery of important interacting proteins and cofactors. G-Biosciences offers a complete kit for the coupling of peptides and proteins to agarose through thei..
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HOOK™ GST Protein Purification kit allows for the purification of soluble, GST tagged protein from bacterial cultures.  The bacteria are first lysed with Bacterial PE LB™ and PE LB™-Lysozyme to release total soluble protein, whilst maintaining the structure and activity of the..
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HOOK™ GST Protein Purification kit allows for the purification of soluble, GST tagged protein from yeast cultures. The yeast are first lysed with Yeast PE LB™ and LongLife™ Zymolyase® to release total soluble protein, whilst maintaining the structure and activity of the pr..
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HOOK™ GST Protein Spin Purification kit allows for the rapid purification of soluble, GST tagged protein from bacterial cultures. The bacteria are first lysed with Bacterial PE LB™ and PE LB™-Lysozyme to release total soluble protein, whilst maintaining the structure and activ..
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HOOK™ GST Protein Spin Purification kit allows for the rapid purification of soluble, GST tagged protein from yeast cultures. The yeast are first lysed with Yeast PE LB™ and LongLife™ Zymolyase® to release total soluble protein, whilst maintaining the structure and activit..
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Biotin, a 244Da vitamin (Vitamin H) molecule, exhibits an extraordinary binding affinity for avidin (Ka=1015 M-1). Biotin and avidin interaction is rapid and once the bond is established it can survive up to 3M guanidine-hydrochloride and extremes of pH. Biotin-avidin bonds can only be reversed by d..
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Model:
Immobilized Biotin Resin is designed for the high affinity chromatography purifications of avidin, streptavidin and Neutravidin protein.  The resin consists of biotin coupled to 6% cross-linked agarose.    Biotin, a 244 Dalton vitamin (Vitamin H) molecule, exhibits an extraordin..
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G-Biosciences Immobilized D-Galactose is for the purification of lectins, galactosidases and other galactose-, N-acetylgalactosamine- or carbohydrate binding molecules.  Specific applications include the purification of galactosidases, C-type lectins, enterotoxins and cholera toxin.   Th..
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G-Biosciences Immobilized Heparin is a ready-to-use purification resin for a wide range of proteins.  The resin consists of 6% cross-linked agarose covalently coupled to heparin through amide bonds.  The coupling chemistry used generates a highly stable purification resin that is stable mo..
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Immobilized Iminobiotin Resin consists of iminobiotin, a cyclic guanido analog of biotin, covalently coupled to 6% crosslinked agarose. The resin allows for the purification of avidin, streptavidin and Neutravidin and their subsequent gentle elution using non-denaturing elution buffers.   The..
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G-Biosciences Immobilized Monomeric Avidin Resin is designed for the simple affinity chromatography purifications of proteins, antibodies and other molecules with a biotin tag.  The resin consists of monomeric subunits of avidin covalently coupled to 6% cross-linked agarose, offering a stable, ..
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