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Affinity Purification Resins and Methods

 

An assortment of affinity purification resins for the separation of both tagged and immunoglobulin molecules. A large selection of affinity coupling chemistries are available to isolate your target molecules including carboxyl containing molecules, nucleic acids, glycoproteins, polyclonal antibodies, oxidized sugars and many more.  Efficient Avidin-Streptavidin and Neutravidin purification is simple and affordable with our immobilized biotin and iminobiotin resins. High affinity biotin tagged protein resins are available along with streptavidin binding and elution buffers and reagents for the quantitation of biotin. Calmodulin resin for the affinity purification of calmodulin binding proteins (CBP), including recombinant proteins with a CBP tag and calmodulin-regulated proteins in eukaryotic cells are available as resin only or in kit form including binding and elution buffers. Immobilized p-Aminophenyl Phosphoryl Choline consists of a phosphoryl choline covalently linked to beaded agarose and is designed for the purification of C-reactive protein from plasma, ascites and other biological fluids. A more economical substitute for Immobilized p-Aminophenyl Phosphoryl Choline, Immobilized O-Phosphorylethanolamine is also available for the purification of C-reactive protein.  A human recomboninant C-reactive protein is also offered. Purify your GST-tagged or His-tagged proteins with GST-tagged or His-tagged protein purification resins or kits.  Isolate proteins of the hemostatic pathways, growth hormones and growth factors, lipoproteins and other physiological markers with immobilized heparin and lectins.  Rapidly purify lectins, galactose-binding molecules, agglutinins, N-acetylgalactosamine-binding, N-acetylglucasomine-binding and carbohydrate binding molecules with our lectin purification reagents. A variety of non-porous, high antibody binding capacity, cost effective magnetic beads are available. Immobilized boronic acid is designed specifically for the simple isolation of small molecular weight compounds that have cis-diol groups such as ribonucleotide and oligonucleotide RNA. 

 
Download the Protein Purification Handbook for a full list of related products
Download the Purification Resins Handbook for Complete Product List 
G-Biosciences’ Ni-NTA Magnetic Beads are 3µm beads designed for the rapid purification of X His-tagged proteins. Ni-NTA Magnetic Beads have nitrilotriacetic acid (NTA) groups with charged nickel covalently bound to the surface dextran of the beads.  Due to the high affinity, Ni-NTA ..
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Immobilized metal affinity chromatography (IMAC) resin utilizing nickel (Ni2+) for the purification of 6x histidine tagged proteins.   This resin binds to six histidine residues (6x His), a common tag used in protein purification. The resin consists of iminodiacetate coupled to 6% cross-linke..
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ρ-Aminobenzamidine Agarose primary application is for the removal and/or purification of trypsin-like proteases.  ρ-aminobenzamidine (PAB) is a synthetic inhibitor of trypsin-like proteases and has been covalently coupled to 6% crosslinked agarose.   ρ-aminobenzamidine agaros..
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Gene/Protein:
G-Biosciences' Protein A Magnetic Beads are Fe3O4 beads coated with dextran to produce highly uniform, 1µm beads. Recombinant Protein A is covalently coupled to the dextran coat to produce an enhanced alternative to agarose slurries for immunoprecipitation experiments.   The use..
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G-Biosciences' Protein G Magnetic Beads are Fe3O4 beads coated with dextran to produce highly uniform, 1µm beads. Recombinant Protein G is covalently coupled to the dextran coat to produce an enhanced alternative to agarose slurries for immunoprecipitation experiments. G-Biosciences m..
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The SDC™ (Steroid/ Drug/ Compound) Immobilization kit is designed for the immobilization of steroids, drugs and chemical compounds that lack primary amines, sulfhydryls, carbonyls and other common coupling groups to a solid-phase agarose support for the use in affinity purification.  The ..
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The Sulfhydryl Immobilization Kit for Peptides is designed for the simple and efficient coupling of sulfhydryl-containing peptides to a solid agarose support. The Sulfhydryl Coupling Resin Columns utilizes iodoacetyl groups that specifically react with free sulfhydryls to form covalent, permanent th..
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The Sulfhydryl Immobilization Kit for Proteins is a complete kit designed for the simple and efficient coupling of proteins to a solid agarose support. The Sulfhydryl Coupling Resin Columns utilizes iodoacetyl groups that specifically react with free sulfhydryls to form covalent, permanent thioether..
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G-Biosciences’ Amine Magnetic Beads are 1µm, uniform magnetic beads with an amine (-NH2) surface functional group. The magnetic beads consist of a single-crystal Fe3O4 sphere core and dextran coating layer. Through chemical modification of dextran, the primary amino group (-NH2) are..
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The Carbohydrate Coupling Resin (Hydrazide resin) is designed for the simple and efficient coupling of glycoproteins to a solid agarose support through oxidized sugar groups. The coupling is through the hydrazide group that is immobilized to agarose beads. The resin is ideal for immobilized polyclon..
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Gene/Protein:
Glutathione Resin is designed for the affinity purification of proteins with a glutathione S-transferase (GST) tag. The resin consists of reduced glutathione (GSH) covalently coupled to 6% cross-linked agarose, via a 10-carbon spacer arm. The resin has a binding capacity of >40mg GST/ml resin. Su..
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The amine reactive HOOK™ Activated Agarose is 6% agarose that has been activated to generate reactive aldehyde groups. The aldehyde groups of the agarose react spontaneously with primary amines, located at the N-terminus of proteins or in lysine residues, to form intermediate Schiff Base compl..
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