Proteomics
Our Protein Research section covers the spectrum of research products for proteomics.
There are products for Protein Purification, including Protein Extraction, Fractionation, Sample Preparation and Protein Concentration.
Our Protein Analysis products cover Protein Electrophoresis, Western Blot, and Mass Spectrometry. A wide selection of Protein Labeling and Protein Modification tools are available for protein binding and protein structure studies.
A large selection of Protein Assays are available, including unique Protein Estimation Assays.
ASB-16 is a zwitterionic detergents composed of a sulfobetaine with a C16 alkyl tail that is highly efficient in solubilizing proteins for 2D electrophoresis analysis and other downstream applications. Optimal solubility is achieved in Urea and thiourea buffers, but not in urea alone.
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Type: Zwitterionic detergent
Full Name: Amidosulfobetaine-14
Molecular Formula: C22H46N2O4S
Molecular Weight: 434.7
Form: White to off white powder
Purity: >99%
Solubility: Water soluble
Conductivity: <50µS in a 10% solu..
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Gene/Protein:
Also known as bovine pancreatic trypsin inhibitor.
Activity: Approx 6,000 KIU/mg
Specificity: A broad range, competitive and reversible inhibitor of chymotrypsin, plasmin, trypsin, kallikrein and other serine proteases
Solubility: Soluble ..
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ρ-Azidophenyl glyoxal monohydrate
Features
Molecular Weight: 193.16
Spacer Arm (Å): 9.3
Reactive Toward: Arginines
Membrane Permeable: YES
Water Soluble: NO
Cleavable/ Reversible: NO
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APDP
A sulfhydryl-reactive and photoreactive cross-linker
Features
Chemical Name: N-[4-(p-Azidosalicylamido)butyl]-3’-(2’-pyridyldithio)propionamide
Synonym: APDP
Formula: C₁₈H₂₀N₆O₂
Molecular Weight: 446.55
Spacer Arm: 21.0Å
Reactive Toward: sulfhydryl and amino..
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Gene/Protein:
[(S)-1-Carboxy-2-Phenyl]-carbamoyl-Arg-Val-arginal
Specificity: Inhibits Ca2+-dependent endopeptidases, including papain, trypsin-like serine proteases, some cysteine proteases and to a lesser extent plasmin. Higher specificity for trypsin and papain compared to leupeptin...
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N-5-Azido-2-nitrobenzyloxysuccinimide Features Molecular Weight: 305.20 Spacer Arm (Å): 7.7 Reactive Toward: Primary Amines Membrane Permeable: YES Water Soluble: NO Cleavable/ Reversible: No..
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Gene/Protein:
Features
CAS#: 631-61-8
Molecular Formula: C2H3O2NH4
Ammonium Acetate Molecular Weight: 77.08
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Calpain inhibitor I; N-[N-(N-Acetyl-L-leucyl)-L-leucyl]-L-norleucine
Specificity: Cell permeable peptide aldehyde inhibitor of calpain I and to a lesser extent calpain II. Also inhibits other neutral cysteine proteases, cathepsin B and L and the proteasome.
Solubility: Solubl..
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Gene/Protein:
Samples that contain a large abundance of albumin, such as plasma and cerebrospinal fluid, tend to mask identification and discovery of other less abundant proteins in two dimensional gel electrophoresis and other studies. AlbuminOUT™ has been specifically developed for substantial removal of ..
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-500x500h.jpg "Agarose Sealing Solution")
Gene/Protein:
Simply heat and use for sealing the IPG strips for SDS-PAGE analysis. The Agarose Sealing Solution is prepared in a proprietary buffer to minimize reoxidation of the competing thiol pairs as proteins enter into the second dimension gel. Improves resolution and prevents streaking of protein spots on ..
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4-(2-Aminoethyl) benzenesulfonyl fluoride hydrochloride
Specificity: Specific irreversible inhibitor of serine proteases, including chymotrypsin, kallikrein, plasmin, thrombin and trypsin. A stable non-toxic alternative to PMSF.
Solubility: Soluble in water
Features
Sy..
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ActiveHOOK™ KLH is maleimide activated by the addition of sulfoSMCC cross-linker. The ActiveHOOK™ KLH rapidly couples to free sulfhydryl groups on peptides and proteins. This activated carrier proteins save both time and money as separate cross-linkers are not required and the ..
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ActiveHOOK™ HyperCarrier™ is maleimide activated HyperCarrier™, by the addition of sulfoSMCC cross-linker. The ActiveHOOK™ HyperCarrier™ rapidly couples to free sulfhydryl groups on peptides and proteins. This activated carrier proteins save both time and mone..
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ActiveHOOK™ BSA is maleimide activated BSA, by the addition of sulfoSMCC cross-linker. The ActiveHOOK™ BSA rapidly couples to free sulfhydryl groups on peptides and proteins. This activated carrier proteins save both time and money as separate cross-linkers are not required and the ..
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ρ-Azidobenzoyl Hydrazide
Features
Molecular Weight: 177.19
Spacer Arm (Å): 11.9
Reactive Toward: Carbohydrates
Membrane Permeable: YES
Water Soluble: NO
Cleavable/ Reversible: NO
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-500x500h.jpg "2D-Xtract™")
Gene/Protein:
A suitable protein solubilization buffer for 2D analysis must solubilize proteins effectively, without disturbing the native charge of the proteins. Urea based solubilization buffers solubilize proteins effectively, however can modify the native
charge of the proteins, due to carbamylation. Ur..
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Many commercial grade detergents contain elevated levels of sulfhydryl oxidizing agents, peroxides, salts and carbonyl compounds (Figure 1). The proteins that are isolated with these detergents are highly susceptible to contaminating peroxides and carbonyls. The peroxides will oxidize proteins and t..
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Many commercial grade detergents contain elevated levels of sulfhydryl oxidizing agents, peroxides, salts and carbonyl compounds (Figure 1). The proteins that are isolated with these detergents are highly susceptible to contaminating peroxides and carbonyls. The peroxides will oxidize proteins and t..
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![1-Fluoro-2,4-dinitrobenzene [DNFB]](https://cdn.gbiosciences.com/cache/DNFB-500x500h.png "1-Fluoro-2,4-dinitrobenzene [DNFB]")
1-Fluoro-2,4-dinitrobenzene [DNFB], commonly known as Sanger’s reagent is a chemical amino acid modifier that reacts with N-terminal amino acid of polypeptides. In Sanger reaction DNFB is used to form dinitrophenyl derivatives for end group determination of proteins and to derivatize primary a..
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