Proteomics
Our Protein Research section covers the spectrum of research products for proteomics.
There are products for Protein Purification, including Protein Extraction, Fractionation, Sample Preparation and Protein Concentration.
Our Protein Analysis products cover Protein Electrophoresis, Western Blot, and Mass Spectrometry. A wide selection of Protein Labeling and Protein Modification tools are available for protein binding and protein structure studies.
A large selection of Protein Assays are available, including unique Protein Estimation Assays.
HOOK™ NHS-Biotin reacts with protein primary amines. Amines, lysine ε-amines and N-terminal α-amines, are the most abundant group in protein molecules and represent the most common target for biotinylation. For example, BSA contains 59 primary amines, of which up to 35 are availa..
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HOOK™ Iodoacetyl-LC-Biotin reacts with protein free sulhydryl groups. Sulfhydryl reactive reagents are more specific and react only with free sulfhydryl residues (-SH or thiol groups). The side chain of the amino acid cysteine is the most common source of free sulfhydryl groups. If free sulfhy..
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The use of biotin for non-radioactive labeling of proteins and nucleic acids has become an increasingly popular technique in life science research. Several factors must be considered when coupling a biotin reagent to a protein in order to ensure a successful reaction. The primary consideration is th..
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The use of biotin for non-radioactive labeling of proteins and nucleic acids has become an increasingly popular technique in life science research. Several factors must be considered when coupling a biotin reagent to a protein in order to ensure a successful reaction. The primary considera..
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HOOK™ Biotin-PDA reacts with protein free sulhydryl groups. Sulfhydryl reactive reagents are more specific and react only with free sulfhydryl residues (-SH or thiol groups). The side chain of the amino acid cysteine is the most common source of free sulfhydryl groups. If free sulfhydryl resid..
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The use of biotin for non-radioactive labeling of proteins and nucleic acids has now become an increasingly popular technique in life science research. Several factors must be considered when coupling a biotin reagent to a protein to ensure a successful reaction. The primary consideration is the sel..
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The use of biotin for non-radioactive labeling of proteins and nucleic acids has now become an increasingly popular technique in life science research. Several factors must be considered when coupling a biotin reagent to a protein to ensure a successful reaction. The primary consideration is the sel..
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HOOK™ Biotin-BMMCC reacts with protein free sulhydryl groups. Sulfhydryl reactive reagents are more specific and react only with free sulfhydryl residues (-SH or thiol groups). The side chain of the amino acid cysteine is the most common source of free sulfhydryl groups. If free sulfhydryl res..
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The use of peptides for the generation of antibodies against specific peptides has become an essential tool in proteomic research.
A crucial step in using peptides as antigens is their coupling to carrier proteins. Peptides are designed to be good epitopes by themselves; however they fail t..
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Gene/Protein:
FITC (fluorescein isothiocyanate) is a commonly used fluorescent label for proteins, as it contains the groups required for conjugating to amino, sulfhydryl, imidazoyl, tyrosyl or carbonyl groups of proteins. FITC has a molecular weight of 389, and excitation and emission wavelengths of 494nm and 52..
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Gene/Protein:
(5/6) TAMRA-SE (5-(and-6)-Carboxytetramethylrhodamine succinimidyl ester, mixed isomers) is based on tetramethylrhodamine, one of the most common fluorophores used in the labeling of peptides, proteins, nucleic acids and nucleotides.
(5/6) TAMRA absorbs green visible light at 546nm and emits an o..
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Gene/Protein:
HOOK™ Cell Surface Protein Isolation kit uses G-Biosciences HOOK™ biotin labeling and purification technology in conjunction with our Mammalian Cell PE LB™ lysis buffer to conveniently label cell surface proteins and isolate them for further analysis, including Western blotting.
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HOOK™ BiotinQuant™ uses HABA (2-(4-Hydroxyphenylazo)benzoic acid/ 2-(4′-Hydroxybenzeneazo)benzoic acid/ 4′-Hydroxyazobenzene-2-carboxylic acid) to rapidly estimate the mole-to-mole ratio of biotin to antibody or protein.
The method of biotin incorporation estimation ..
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The amine reactive HOOK™ Activated Agarose is 6% agarose that has been activated to generate reactive aldehyde groups. The aldehyde groups of the agarose react spontaneously with primary amines, located at the N-terminus of proteins or in lysine residues, to form intermediate Schiff Base compl..
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An important tool in research is the generation of peptide and protein affinity columns for the purification of antibodies and for the discovery of important interacting proteins and cofactors. G-Biosciences offers a complete kit for the coupling of peptides and proteins to agarose through thei..
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![High Molecular Weight Transfer Buffer [5X]](https://cdn.gbiosciences.com/cache/JPEG_786-423(NEW)-500x500h.jpg "High Molecular Weight Transfer Buffer [5X]")
Based on Efficient™ Western Transfer Buffer, the High Molecular Weight Transfer buffer is designed to facilitate the transfer of notoriously difficult high molecular weight proteins (>70kDa) during Western blotting.
Supplied as 1 liter of a 5X concentrated solution...
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Gene/Protein:
A selection of strong chemicals to aid in the denaturation of proteins. Denaturants, or chaotropes, disrupt water interactions resulting in the solubilization of hydrophobic proteins and peptides. The chaotropes also act on all proteins to unfold them and alter their three-dimensional structure.
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N-γ-Maleimidobutyryloxysuccinimide ester
Features
Molecular Weight: 280.23
Spacer Arm (Å): 6.8
Reactive Toward: Primary Amine + Sulfhydryl
Membrane Permeable: YES
Water Soluble: NO
Cleavable/ Reversible: NO
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Gene/Protein:
For the highly sensitive detection of glycoproteins following gel electrophoresis (Figure 1) or protein transfer to nitrocellulose membranes (Figure 2).
The kit uses an enhanced Periodic Acid-Schiff (PAS) method for detection of glycoprotein sugars. The supplied oxidizing agent fi..
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Gene/Protein:
Glutathione Resin is designed for the affinity purification of proteins with a glutathione S-transferase (GST) tag. The resin consists of reduced glutathione (GSH) covalently coupled to 6% cross-linked agarose, via a 10-carbon spacer arm. The resin has a binding capacity of >40mg GST/ml resin. Su..
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Gene/Protein:
Spray and Wipe; Specifically Formulated For Electrophoresis Gel Plates
Clean your electrophoresis plates with GELPLATE-clean™ and prevent poor electrophoretic band migration, band distortions, and poor image development. Simply spray and wipe clean your electrophoresis plates with..
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G-Trap™ SP Agarose Fast Flow is ready to use prepacked column used for seperation of proteins and other biomolecules based on charge. The resin in G-Trap™ SP Agarose Fast Flow is a strong cation exchanger composed of highly crosslinked 6% agarose beads coupled with sulphopropyl (SP)..
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G-Trap™ rProtein A FF are prepacked ready to use columns for purification of IgG antibodies.
The column is packed with fast flow resin, which is covalently coupled to recombinant Protein A. Recombinant Protein A is coupled covalently by proprietary coupling method to highly crosslinke..
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G-Trap™ Q Agarose Fast Flow is ready to use prepacked column used for seperation of proteins and other biomolecules based on charge. The resin in G-Trap™ Q Agarose Fast Flow is a strong anion exchanger composed of highly crosslinked 6% agarose beads coupled with quaternary ammonium ..
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