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Affinity Purification Resins and Methods

 

An assortment of affinity purification resins for the separation of both tagged and immunoglobulin molecules. A large selection of affinity coupling chemistries are available to isolate your target molecules including carboxyl containing molecules, nucleic acids, glycoproteins, polyclonal antibodies, oxidized sugars and many more.  Efficient Avidin-Streptavidin and Neutravidin purification is simple and affordable with our immobilized biotin and iminobiotin resins. High affinity biotin tagged protein resins are available along with streptavidin binding and elution buffers and reagents for the quantitation of biotin. Calmodulin resin for the affinity purification of calmodulin binding proteins (CBP), including recombinant proteins with a CBP tag and calmodulin-regulated proteins in eukaryotic cells are available as resin only or in kit form including binding and elution buffers. Immobilized p-Aminophenyl Phosphoryl Choline consists of a phosphoryl choline covalently linked to beaded agarose and is designed for the purification of C-reactive protein from plasma, ascites and other biological fluids. A more economical substitute for Immobilized p-Aminophenyl Phosphoryl Choline, Immobilized O-Phosphorylethanolamine is also available for the purification of C-reactive protein.  A human recomboninant C-reactive protein is also offered. Purify your GST-tagged or His-tagged proteins with GST-tagged or His-tagged protein purification resins or kits.  Isolate proteins of the hemostatic pathways, growth hormones and growth factors, lipoproteins and other physiological markers with immobilized heparin and lectins.  Rapidly purify lectins, galactose-binding molecules, agglutinins, N-acetylgalactosamine-binding, N-acetylglucasomine-binding and carbohydrate binding molecules with our lectin purification reagents. A variety of non-porous, high antibody binding capacity, cost effective magnetic beads are available. Immobilized boronic acid is designed specifically for the simple isolation of small molecular weight compounds that have cis-diol groups such as ribonucleotide and oligonucleotide RNA. 

 
Download the Protein Purification Handbook for a full list of related products
Download the Purification Resins Handbook for Complete Product List 
Gene/Protein:
Adenosine 2′,5′-diphosphate is NADP analog. Immobilized Adenosine 2′,5′-Diphosphate is Adenosine 2′,5′-diphosphate covalently bound to agarose via a spacer. It is used in purification of NADP-dependent enzymes   Click here to request bulk or custom size..
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Gene/Protein:
Immobilized ADP is used in purification of heat shock proteins.     Click here to request bulk or custom sizes.   Features: ADP coupled to 6% cross-linked Agarose Extent of labeling is 1- 5 µmol ADP per ml resin  ​Shipped as hydrated resin in..
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Gene/Protein:
Immobilized AMP is an affinity resin used in purifying NAD+-dehydrogenases such as aldehyde dehydrogenase, NADH oxidase and Lactate Dehydrogenase Isoenzyme-5, and ATP-dependent enzymes such as DNA polymerase.   Click here to request bulk or custom sizes. Features: AMP coupled to ..
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Gene/Protein:
Immobilized Anti-BSA Antibody is prepared by covalent bonding of Anti-BSA Antibody produced in rabbit with 6% cross-linked agarose beads. It is used for immunoprecipitation and immunoaffinity purification of BSA from solutions. Rabbit Anti-Bovine Albumin (BSA)-Agarose antibody is also used as a nega..
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Gene/Protein:
Immobilized ATP is used for purifying ATP-binding proteins and enzymes such as cyclin-dependent kinases, heat shock proteins and cryptochromes.   Click here to request bulk or custom sizes. Features: ATP coupled to 6% cross-linked Agarose Extent of labeling is 1- 5 µ..
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Immobilized Biotin Resin is designed for the high affinity chromatography purifications of avidin, streptavidin and Neutravidin protein.  The resin consists of biotin coupled to 6% cross-linked agarose.    Biotin, a 244 Dalton vitamin (Vitamin H) molecule, exhibits an extraordinary ..
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Gene/Protein:
Immobilized Catalase is prepared by covalent coupling of catalase enzyme to 6% cross-linked agarose beads. Immobilized Catalase is used to remove hydrogen peroxide from biological samples for use in downstream applications which require absenceof hydrogen peroxide as it causes interference. Immob..
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Gene/Protein:
Immobilized Cibacron Blue 3G is prepared by covalent linking of Cibacron Blue 3G dye with 6% cross-linked agarose. The resin is offered based on property of textile dyes to bind proteins, especially those with affinities to various nucleotides. Furthermore, affinity of reactive dyes to proteins..
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Gene/Protein:
Immobilized Coenzyme A is made by covalently coupling Coenzyme A with 6% cross linked agarose beads. Immobilized Coenzyme A is used in affinity purification of Coenzyme A binding enzymes.   Click here to request bulk or custom sizes. Features: Coenzyme A coupled to 6% cross-linke..
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Gene/Protein:
Immobilized D-Mannose is prepared by covalent coupling of D-mannose with 6% cross-linked agarose beads. It is used in affinity purification of mannose- binding proteins such as lectin concanavalin A.   Click here to request bulk or custom sizes.   Features D-Mannose coupl..
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Gene/Protein:
Immobilized DNase I is designed for use in molecular biology to remove DNA from RNA samples for RT-PCR purposes. Some RT-PCR reactions require removal of DNA from samples to prevent their interference in RT-PCR reactions. Samples can be directly treated with DNase I, however this adds addition step ..
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Gene/Protein:
Immobilized Dye Resin Selection Kit is designed based on the property of textile dyes binding to proteins, especially those with affinities to various nucleotides. Furthermore, the affinity of reactive dyes to proteins may be due to substrate/cofactor similarities as well as hydrophobic and ion exch..
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