Proteomics
Our Protein Research section covers the spectrum of research products for proteomics.
There are products for Protein Purification, including Protein Extraction, Fractionation, Sample Preparation and Protein Concentration.
Our Protein Analysis products cover Protein Electrophoresis, Western Blot, and Mass Spectrometry. A wide selection of Protein Labeling and Protein Modification tools are available for protein binding and protein structure studies.
A large selection of Protein Assays are available, including unique Protein Estimation Assays.
Gene/Protein:
Extracts and solubilizes nearly all of the proteins from mammalian samples, including membrane as well as soluble proteins, by a strong chaotropic extraction buffer to solubilize even the most difficult proteins. Suitable for biological samples from animal tissues and adherent and suspension c..
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Gene/Protein:
FOCUS™ Membrane Proteins is a rapid and highly reproducible method for preparation of membrane or hydrophobic proteins from biological samples for 2D-gel analysis or other applications. Membrane proteins are extracted with a single step phase partition, with an efficiency greater than 90% with..
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Protocols.io provides an interactive version of this protocol where you can discover and share optimizations with the research community.
Specifically designed for the isolation of intact mitochondria from cultured mammalian cells. This kit allows for the fast and efficient fractionation of t..
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FOCUS™ PhosphoRich™ is a ready-to-use kit that enriches phosphorylated proteins and phosphopeptides from complex biological samples. The kit contains spin columns that have a phosphoprotein binding resin with a binding capacity of ~20mg phosphorylated ovalbumin per column. The resin colu..
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Specifically designed for plant research and supplied with plant specific reagents, including reagents for removal of plant pigments and other natural products that may interfere with protein analysis. Extracts and solubilizes nearly all of the proteins from plants, including membrane as well as sol..
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The kit is supplied with a proprietary buffer necessary for efficient alkylation of thiols, while minimizing reoxidation of the competing thiol pairs in protein samples. Simply add an appropriate amount to reagent solutions for alkylation of protein thiol groups. Features Pro..
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A water soluble, odorless, non-toxic and stable TCEP [Tris (2-carboxyethyl) phosphine] reductant for protein reduction.
FOCUS™ Protein Reductant has improved stability and efficiency compared to DTT and reduces proteins over a wide range of pH, including lower acidic pH. FOCUS™ ..
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The FOCUS™ Reduction-Alkylation kit offers a simple two-step method for reduction and alkylation of protein samples for 2D gel analysis. The disulfide bonds are reduced with a highly reactive and stable TCEP [Tris (2-carboxyethyl) phosphine] followed by blocking of the thiols by alkyl..
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Gene/Protein:
Lipid rafts are membrane microdomains that are enriched in caveolin, cholesterol, glycolipids, sphingolipids and glycosyl-phosphatidylinositol. Lipid rafts are also known as detergent-insoluble glycolipid-enriched complexes (GEMs) or DIGs. Many signaling proteins, including glycosylphosphatidylinosi..
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Gene/Protein:
A complete kit for the selective preparation of soluble (hydrophilic) and insoluble (hydrophobic) proteins from mammalian tissues and cells, plants, yeast, bacteria, and other biological samples. The kit comes with reagents necessary for fractionation of soluble and insoluble fractions, including a ..
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Gene/Protein:
Protocols.io provides an interactive version of this protocol where you can discover and share optimizations with the research community.
FOCUS™ SubCell is designed for the total subcellular fractionation of cells and mammalian tissue into enriched fractions of nuclear, mitochondr..
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Gene/Protein:
Specifically designed for yeast research and supplied with yeast specific reagents. Extracts and solubilizes nearly all of the proteins from yeast, including membrane and soluble proteins. Extraction is based on gentle lysis of yeast cells with a yeast lytic enzyme preparation, LongLife™..
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Gene/Protein:
2D electrophoresis and mass spectrometry is routinely used for identification of novel proteins, however the greatest challenge in protein identification is achieving suitable resolution of proteins. The high dynamic range of a species' proteome means that the more abundant proteins mask the les..
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Gene/Protein:
Electroelution of nucleic acids and proteins has many advantages as it avoids centrifugation, vortexing, heating, precipitation and allows minimal manipulation of samples. Electroelution normally involves dialysis tubing, which results in extreme dilution of precious samples. G-Capsule™ is a s..
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G-Trap™ Butyl Agarose 6 Fast Flow are prepacked ready to use columns for seperation of biomolecules via Hydrophobic Interaction chromatography (HIC).
Biomolecules are separated on the HIC column based upon the hydrophobicity of the ligand attached to the resin, the distribution ..
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G-Trap™ CM Agarose Fast Flow is ready to use prepacked column used for separation of proteins and other biomolecules based on charge. The resin in G-Trap™ CM Agarose Fast Flow is a weak cation exchanger composed of highly crosslinked 6% agarose beads coupled with carboxymethyl (CM) weak ..
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G-Trap™ Co IDA Agarose Fast Flow columns are ready to use prepacked affinity columns used for purification of proteins based on immobilized metal ion affinity chromatography (IMAC).
IMAC is based on interaction of cations of transition metals such as Ni2+, Co2+ with protein residues h..
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Gene/Protein:
G-Trap™ GT-100 Desalting Column is prepacked ready to use column for separation of low molecular weight substances from high molecular weight substances based on size exclusion chromatography.
G-Trap™ GT-100 Desalting Columns are mostly used for removal of salt or buff..
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Gene/Protein:
G-Trap™ GT-600 Desalting Column is prepacked ready to use column for separation of low molecular weight substances from high molecular weight substances based on size exclusion chromatography.
G-Trap™ GT-600 Desalting Columns are mostly used for removal of salt or buff..
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G-Trap™ HIC Selection Kit consists of 4 prepacked 1 ml columns (G-Trap™ HIC Columns) with matrix or resin for hydrophobic interaction chromatography. The kit consists of 4 pepacked columns namely G-Trap™ Butyl Agarose 6 Fast Flow, G-Trap™ Octyl Agarose 6 Fast Flow, G-Trap&tra..
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G-Trap™ Ion Exchange Selection Kit contains 4 different types of 1 ml G-Trap™ Ion Exchange Agarose Fast Flow Columns which are used for initial screening of the suitable ion exchange resin and/or pH or ionic strength optimization of start and elution buffer to separate the target protein..
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G-Trap™ Ni IDA Agarose Fast Flow columns are ready to use prepacked affinity columns used for purification of proteins based on immobilized metal ion affinity chromatography (IMAC).
IMAC is based on interaction of cations of transition metals such as Ni2+, Co2+ with protein resid..
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G-Trap™ Octyl Agarose 6 Fast Flow are prepacked ready to use columns for seperation of biomolecules via Hydrophobic Interaction chromatography (HIC).
Biomolecules are separated on the HIC column based upon the hydrophobicity of the ligand attached to the resin, the distribution ..
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G-Trap™ Q Agarose Fast Flow is ready to use prepacked column used for seperation of proteins and other biomolecules based on charge. The resin in G-Trap™ Q Agarose Fast Flow is a strong anion exchanger composed of highly crosslinked 6% agarose beads coupled with quaternary ammonium ..
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G-Trap™ SP Agarose Fast Flow is ready to use prepacked column used for seperation of proteins and other biomolecules based on charge. The resin in G-Trap™ SP Agarose Fast Flow is a strong cation exchanger composed of highly crosslinked 6% agarose beads coupled with sulphopropyl (SP)..
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Gene/Protein:
Spray and Wipe; Specifically Formulated For Electrophoresis Gel Plates
Clean your electrophoresis plates with GELPLATE-clean™ and prevent poor electrophoretic band migration, band distortions, and poor image development. Simply spray and wipe clean your electrophoresis plates with..
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Gene/Protein:
For the highly sensitive detection of glycoproteins following gel electrophoresis (Figure 1) or protein transfer to nitrocellulose membranes (Figure 2).
The kit uses an enhanced Periodic Acid-Schiff (PAS) method for detection of glycoprotein sugars. The supplied oxidizing agent fi..
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N-γ-Maleimidobutyryloxysuccinimide ester
Features
Molecular Weight: 280.23
Spacer Arm (Å): 6.8
Reactive Toward: Primary Amine + Sulfhydryl
Membrane Permeable: YES
Water Soluble: NO
Cleavable/ Reversible: NO
..
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Gene/Protein:
A selection of strong chemicals to aid in the denaturation of proteins. Denaturants, or chaotropes, disrupt water interactions resulting in the solubilization of hydrophobic proteins and peptides. The chaotropes also act on all proteins to unfold them and alter their three-dimensional structure.
..
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![High Molecular Weight Transfer Buffer [5X]](https://cdn.gbiosciences.com/cache/JPEG_786-423(NEW)-500x500h.jpg "High Molecular Weight Transfer Buffer [5X]")
Based on Efficient™ Western Transfer Buffer, the High Molecular Weight Transfer buffer is designed to facilitate the transfer of notoriously difficult high molecular weight proteins (>70kDa) during Western blotting.
Supplied as 1 liter of a 5X concentrated solution...
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An important tool in research is the generation of peptide and protein affinity columns for the purification of antibodies and for the discovery of important interacting proteins and cofactors. G-Biosciences offers a complete kit for the coupling of peptides and proteins to agarose through thei..
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HOOK™ BiotinQuant™ uses HABA (2-(4-Hydroxyphenylazo)benzoic acid/ 2-(4′-Hydroxybenzeneazo)benzoic acid/ 4′-Hydroxyazobenzene-2-carboxylic acid) to rapidly estimate the mole-to-mole ratio of biotin to antibody or protein.
The method of biotin incorporation estimation ..
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Gene/Protein:
HOOK™ Cell Surface Protein Isolation kit uses G-Biosciences HOOK™ biotin labeling and purification technology in conjunction with our Mammalian Cell PE LB™ lysis buffer to conveniently label cell surface proteins and isolate them for further analysis, including Western blotting.
..
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Gene/Protein:
(5/6) TAMRA-SE (5-(and-6)-Carboxytetramethylrhodamine succinimidyl ester, mixed isomers) is based on tetramethylrhodamine, one of the most common fluorophores used in the labeling of peptides, proteins, nucleic acids and nucleotides.
(5/6) TAMRA absorbs green visible light at 546nm and emits an o..
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Gene/Protein:
FITC (fluorescein isothiocyanate) is a commonly used fluorescent label for proteins, as it contains the groups required for conjugating to amino, sulfhydryl, imidazoyl, tyrosyl or carbonyl groups of proteins. FITC has a molecular weight of 389, and excitation and emission wavelengths of 494nm and 52..
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The use of peptides for the generation of antibodies against specific peptides has become an essential tool in proteomic research.
A crucial step in using peptides as antigens is their coupling to carrier proteins. Peptides are designed to be good epitopes by themselves; however they fail t..
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HOOK™ Biotin-BMMCC reacts with protein free sulhydryl groups. Sulfhydryl reactive reagents are more specific and react only with free sulfhydryl residues (-SH or thiol groups). The side chain of the amino acid cysteine is the most common source of free sulfhydryl groups. If free sulfhydryl res..
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The use of biotin for non-radioactive labeling of proteins and nucleic acids has now become an increasingly popular technique in life science research. Several factors must be considered when coupling a biotin reagent to a protein to ensure a successful reaction. The primary consideration is the sel..
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The use of biotin for non-radioactive labeling of proteins and nucleic acids has now become an increasingly popular technique in life science research. Several factors must be considered when coupling a biotin reagent to a protein to ensure a successful reaction. The primary consideration is the sel..
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HOOK™ Biotin-PDA reacts with protein free sulhydryl groups. Sulfhydryl reactive reagents are more specific and react only with free sulfhydryl residues (-SH or thiol groups). The side chain of the amino acid cysteine is the most common source of free sulfhydryl groups. If free sulfhydryl resid..
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The use of biotin for non-radioactive labeling of proteins and nucleic acids has become an increasingly popular technique in life science research. Several factors must be considered when coupling a biotin reagent to a protein in order to ensure a successful reaction. The primary considera..
$0.00
The use of biotin for non-radioactive labeling of proteins and nucleic acids has become an increasingly popular technique in life science research. Several factors must be considered when coupling a biotin reagent to a protein in order to ensure a successful reaction. The primary consideration is th..
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HOOK™ Iodoacetyl-LC-Biotin reacts with protein free sulhydryl groups. Sulfhydryl reactive reagents are more specific and react only with free sulfhydryl residues (-SH or thiol groups). The side chain of the amino acid cysteine is the most common source of free sulfhydryl groups. If free sulfhy..
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HOOK™ NHS-Biotin reacts with protein primary amines. Amines, lysine ε-amines and N-terminal α-amines, are the most abundant group in protein molecules and represent the most common target for biotinylation. For example, BSA contains 59 primary amines, of which up to 35 are availa..
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HOOK™-NHS-LC-Biotin reacts with protein primary amines. Amines, lysine ε-amines and N-terminal α-amines, are the most abundant group in protein molecules and represent the most common target for biotinylation. For example, BSA contains 59 primary amines, of which up to 35 a..
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HOOK™ NHS-LC-LC-Biotin reacts with protein primary amines. Amines, lysine ε-amines and N-terminal α-amines, are the most abundant group in protein molecules and represent the most common target for biotinylation. For example, BSA contains 59 primary amines, of which up to 3..
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HOOK™ NHS-SS-Biotin reacts with protein primary amines. Amines, lysine ε-amines and N-terminal α-amines, are the most abundant group in protein molecules and represent the most common target for biotinylation. For example, BSA contains 59 primary amines, of which up to 35 a..
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HOOK™ PEG2-Iodoacetyl-Biotin reacts with protein free sulhydryl groups.
Sulfhydryl reactive reagents are more specific and react only with free sulfhydryl residues (-SH or thiol groups). The side chain of the amino acid cysteine is the most common source of free sulfhydryl groups.
If..
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HOOK™ PFP-Biotin reacts with protein primary amines. Amines, lysine ε-amines and N-terminal α-amines, are the most abundant group in protein molecules and represent the most common target for biotinylation. For example, BSA contains 59 primary amines, of which up to 35 are ..
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The use of biotin for non-radioactive labeling of proteins and nucleic acids has now become an increasingly popular technique in life science research. Several factors must be considered when coupling a biotin reagent to a protein to ensure a successful reaction. The primary consideration is the sel..
$0.00
HOOK™ Sulfo-NHS-Biotin reacts with protein primary amines. Amines, lysine ε-amines and N-terminal α-amines, are the most abundant group in protein molecules and represent the most common target for biotinylation. For example, BSA contains 59 primary amines, of which up to 3..
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Gene/Protein:
HOOK™-Sulfo-NHS-LC-Biotin reacts with protein primary amines. Amines, lysine ε-amines and N-terminal α-amines, are the most abundant group in protein molecules and represent the most common target for biotinylation. For example, BSA contains 59 primary amines, of which up t..
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HOOK™ Sulfo-NHS-LC-LC-Biotin reacts with protein primary amines. Amines, lysine ε-amines and N-terminal α-amines, are the most abundant group in protein molecules and represent the most common target for biotinylation. For example, BSA contains 59 primary amines, of which u..
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Gene/Protein:
HOOK™ Sulfo-NHS-SS-Biotin reacts with protein primary amines. Amines, lysine ε-amines and N-terminal α-amines, are the most abundant group in protein molecules and represent the most common target for biotinylation. For example, BSA contains 59 primary amines, of which up t..
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HyperCarrier™ is normal BSA that has been treated with ethylene diamine, which substitutes anionic carboxyl groups with cationic aminoethyl-amide groups.
Researchers were able to demonstrate that a cationized form of BSA, produced by replacing anionic side chain carboxylic groups with..
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The expression of recombinant proteins is a popular and routinely used technique in protein studies. The expression of recombinant proteins often has one drawback and that is the recombinant proteins aggregate and form inclusion bodies, especially when expressed at high levels. The aggre..
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-500x500h.jpg "IBS™ Buffer")
Gene/Protein:
The expression of recombinant proteins is a popular and routinely used technique in protein studies. The expression of recombinant proteins often has one drawback and that is the recombinant proteins aggregate and form inclusion bodies, especially when expressed at high levels. The aggre..
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-500x500h.jpg "Immobilized Protein G")
Gene/Protein:
Immobilized protein G is for binding the constant domains of immunoglobulin (Ig) molecules (see Figure 1). It is ideal for the easy one-step purification of classes, subclasses and fragments of immunoglobulins for biological fluids and cell culture media. Protein G is a modified form of Streptococca..
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-500x500h.jpg "Immobilized Streptavidin Resin")
Biotin, a 244Da vitamin (Vitamin H) molecule, exhibits an extraordinary binding affinity for avidin (Ka=10¹âµM-¹) and streptavidin (Ka=10¹âµM-¹). Biotin and (strept)avidin interaction is rapid. Once the bond is established, it can survive up to 3M guanid..
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Gene/Protein:
Immobilized Trypsin is TPCK Treated Trypsin immobilized on 4% agarose that eliminates the contamination of protein digests by the trypsin. The immobilized trypsin is readily removed by separating the agarose from the digestion solution.
Trypsin is a serine endopeptidase that spe..
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A 96-well format kit suitable for processing larger numbers of protein spots concurrently and compatible with spot-picking instruments. Fewer than 96 samples a batch may also be processed without any waste.
Excise protein spots and transfer to the supplied proteomic grade titer plate, des..
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The InGel™ Blue kit provides a complete set of reagents for the in gel tryptic digestion and extraction of peptides for mass spectrometry (MALDI and LC MS/MS). The kit is specifically designed for use with Coomassie or fluorescent stained protein spots/bands.
The kit has all th..
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A reliable method for the proteolytic digestion and subsequent extration of silver stained proteins in gel for subsequent analysis by mass spectrometry.
The protein spots are first excised from the gel and transferred to a proteomic grade tube. Silver stained gel pieces are washed with Sil..
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-500x500h.jpg "Insect PE LB™")
Insect Cell-PE LB™ has been developed for extraction of cytoplasmic soluble protein from insect cultured cells. The Insect Cell-PE LB™ is based on organic buffering agents, which utilizes a mild non-ionic detergent, and a proprietary combination of various salts and agents to enhance ext..
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A proteomic grade iodoacetamide in two different quantities. Iodoacetamide is supplied in 5gm bulk quantities or in the OneQuant™ format.
OneQuant™ Iodoacetamide
OneQuant™ iodoacetamide are single aliquots of iodoacetamide that eliminate the need for weighing, preventing ..
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The blot is prepared using total protein extracted from a variety of species, where whole species is not available the whole liver lysate is used. These blots can be used to identify similar proteins in other species, including human, chicken, frog, worm, drosophila, yeast, aspergillus, E. col..
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Keyhole limpet hemocyanin is a widely used carrier protein due to its large molecular mass (4.5x105-1.3x107Da aggregates) and large number of primary amines that can react with cross-linkers for the coupling of peptides. The KLH is supplied as a clear to slightly hazy, bluish solution in phosphate b..
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Features CAS#: 10034-99-8 Molecular Formula: MgSO4.7H2O Molecular Weight: 246.48..
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Maleimide PEG succinimidyl carboxymethyl
Features
Molecular Weight: ~3400
Reactive Toward: Primary Amine + Sulfhydryl
Membrane Permeable: Not tested
Water Soluble: NO
Cleavable/ Reversible: NO
..
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m-Maleimidobenzoyl-N-hydroxysuccinimide ester
Ideal for hapten-carrier protein, toxin-antibody, enzyme-antibody crosslinking
Features
Molecular Weight: 314.25
Spacer Arm (Å): 9.9
Reactive Toward: Primary Amine + Sulfhydryl
Membrane Permeable: YES
..
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Type: Non-ionic detergent
Full Name: N-Decanoyl-N-methylglucamine
Molecular Formula: C17H35NO6
Mol Weight: 349..46
Form: White powder
Purity: >99%
Solubility: Water soluble
Critical micelle concentration (CMC): 6-7mM (25°C)
..
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Type: Non-ionic detergent
Full Name: Octanoyl-N-methylglucamide
Molecular Formula: C15H31NO6
Molecualr Weight: 321.4
Form: White powder
Purity: >99%
Solubility: Water soluble
Critical micelle concentration (CMC): 58mM (25°C)
&nbs..
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Type: Non-ionic detergent
Full Name: Nonaoyl-N-methylglucamide
Mol. Formula: C16H33NO6
Mol Weight: 335.4
Form: White powder
Purity: >99%
Solubility: Water soluble
Critical micelle concentration (CMC): 19-25mM (25°C)
Appl..
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Gene/Protein:
For user's convenience, we offer pre-cut transfer membranes and padding for Western blot transfer procedures. Pre-cut membranes are supplied sandwiched between blotting paper padding. Simply soak the membrane in transfer buffer and assemble with the gel in a transfer cassette. Nitrocellulose, (0..
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