Proteomics
Our Protein Research section covers the spectrum of research products for proteomics.
There are products for Protein Purification, including Protein Extraction, Fractionation, Sample Preparation and Protein Concentration.
Our Protein Analysis products cover Protein Electrophoresis, Western Blot, and Mass Spectrometry. A wide selection of Protein Labeling and Protein Modification tools are available for protein binding and protein structure studies.
A large selection of Protein Assays are available, including unique Protein Estimation Assays.
For dialysis of small sample volumes, equilibrium dialysis, dialysis of single use preparations, and other dialysis applications. The Micro Dialysis Cup has dialysis buffer capacity of 2-15 ml...
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Gene/Protein:
A Grinding Resin For Proteomics & Molecular Biology Applications
Molecular Grinding Resin™ has been specifically developed for effectively grinding small biological samples for the preparation and extraction of DNA, RNA and proteins. The resin consists of high tensile micro-particles, w..
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N-Hydroxysuccinimidyl-4-azidosalicyclic acid
Features
Molecular Weight: 276.21
Spacer Arm (Å): 8.0
Reactive Toward: Primary Amines
Membrane Permeable: YES
Water Soluble: NO
Cleavable/ Reversible: NO
..
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-500x500h.jpg "Nickel Chelating Resin")
Gene/Protein:
Immobilized metal affinity chromatography (IMAC) resin utilizing nickel (Ni2+) for the purification of 6x histidine tagged proteins.
This resin binds to six histidine residues (6x His), a common tag used in protein purification. The resin consists of iminodiacetate coupled to 6% cross-l..
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Type: Non-detergent sulfobetaine
Full Name: 3-(1-Pyridino)-1-propane sulfonate
Molecular Formula: C8H11NO3S
Molecular Weight: 201.4
Form: White Powder
Purity: >99%
Solubility: Water
Applications
ND SB 201 is a Zwitterionic ..
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Type: Non-ionic detergent
Full Name: N-Octyl-beta-D-glucopyranoside
Molecular Formula: C14H28O6
Molecular Weight: 292.4
Form: White to off white powder
Purity: >98%
Solubility: Water soluble
Critical micelle concentration (CMC): 20-25..
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Stabilized ultra sensitive luminol and 1,2 dioxetane based horseradish peroxidase or alkaline phosphatase substrate for the detection of HRP or AP-conjugated antibodies during ELISA.
OptiBlaze™ ELISA femto-HRP or OptiBlaze™ ELISA femto-AP are chemiluminescent detection systems for Enzym..
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Gene/Protein:
The "Critical Micelle Concentration" (CMC) of a detergent varies with temperature, pH, ionic strength, detergent concentration, purity and presence of organic agents in the detergent. Using a large excess of detergent may pose problems during purification procedures or other downstream app..
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The OrgoSol-PROTEIN-Concentration™ kit precipitates protein with a proprietary solvent buffer, OrgoSol™. The OrgoSol™ buffer has been specifically developed for efficient precipitation of protein solutions with minimal disruption to the protein structure and therefore maintains the..
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HPG (p-hydroxyphenylglyoxal) reacts to specifically modify arginine residues under mild conditions to yield spectrophotometrically measurable signal for amino acid detection.
Features of HPG:
• Arginine-specific—reacts specifically with arginine residues under mild conditions (p..
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Gene/Protein:
Remove Electrophoretic Contaminants for Improved Protein Resolution and Publication Quality Gels; Ideal for Difficult or Dilute Samples
Many lysis buffers and reagents used in sample preparation are incompatible with routinely used electrophoretic analysis. The presence of contaminants,..
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Gene/Protein:
PAGEmark™ Blue PLUS Protein Markers
A blue protein standard with 12 prestained proteins covering a wide range of molecular weights: 10-240kDa in Tris-Glycine buffer and 9-235kDa in Bis-Tris (MOPS) buffer or Bis-Tris (MES) buffer.
Proteins are covalently coupled with a..
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Following in gel digestion of proteins with proteases, the addition of Pep-Extract™ rapidly elutes digested peptides in under 20 minutes. The eluted peptides can be used directly in mass spectrometry analysis, without any additional clean up. Supplied with enough reagents for >5..
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Gene/Protein:
Protein samples loaded on iso-electric focusing (IEF) gels should ideally have low conductivity and be free from agents known to interfere with net protein charge. Protein samples containing interfering agents, including ionic detergents, metal ions, salts, lipids, charged polysaccharides, peptides,..
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PhosphoQuant™ is specifically designed for quick and reliable determination of whether a purified protein is phosphorylated and the extent of phosphorylation. The assay is based on the alkaline hydrolysis of phosphates from seryl and threonyl residues in phosphoproteins and the subsequent quantifica..
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Gene/Protein:
Protocols.io provides an interactive version of this protocol where you can discover and share optimizations with the research community.
picoLUCENT™ PLUS-HRP kit is based on our ultra sensitive Luminol substrate that produces chemiluminescence upon reaction with horseradish..
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N-(ρ-Maleimidophenyl) isocyanate
Features
Molecular Weight: 214.18
Spacer Arm (Å): 8.7
Reactive Toward: Sulfhydryl + Hydroxyl
Membrane Permeable: Not tested
Water Soluble: NO
Cleavable/ Reversible: NO
..
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Features
Synonym: Kacac
CAS#: 127-8-2
Molecular Formula: C2H3O2K
Molecular Weight: 98.15..
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Features
Synonym: Caustic potash
CAS#: 1310-58-3
Molecular Formula: KOH
Molecular Weight: 56.11..
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-500x500h.jpg "Protease Assay Kit")
Gene/Protein:
The Protease Activity Assay Kit is designed for the quantitative determination of proteases present in a protein sample, using a dye-labeled protein substrate.
The proteases present in the sample of interest will digest the protein substrate and release dye labeled peptides. The absorbance of the..
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-500x500h.jpg "Protease Assay Substrates")
Gene/Protein:
RESORUFIN-CASEIN PROTEASE SUBSTRATE
A colorimetric substrate that when treated with proteases releases resorufin that has an absorbance of 570nm. Supplied lyophilized.
FITC-CASEIN PROTEASE SUBSTRATE
A fluorescent (fluorescein isothiocyanate (FITC)) substrate that when ..
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Sensitive protease screening is needed to study protease activity present in the sample of interest. The Protease Screening Kit provides you with a simple and quick method for testing your samples for proteolysis.
Simply incubate your sample in the reagent provided and obtain results. The screening..
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Gene/Protein:
Rapidly, mix 1µl protein sample with the supplied Dilution Buffer and apply 1µl of the solution to the test strip by point-of-contact capillary action. Under the assay's specific buffer conditions the protein enters into the matrix of the test strip, binds and saturates as protein di..
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Gene/Protein:
A water soluble, odorless, non-toxic and stable protein reductant. Protein-S-S-Reductant™ uses TCEP (Tris [2-carboxyethyl] phosphine), a popular alternative to ß-mercaptoethanol and DTT (dithiothreitol). TCEP improves stability, increases effectiveness, and reduces proteins over a wider ..
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Gene/Protein:
Due to supply constraints, please request a quote for all Proteinase K items
Proteinase K (also protease K, endopeptidase K, peptidase K or Tritirachium alkaline phosphatase) (EC 3.4.21.64) is a non-specifc, broad spectrum serine protease that is isolated from the saprophytic fung..
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Many commercial grade detergents contain elevated levels of sulfhydryl oxidizing agents, peroxides, salts and carbonyl compounds. The proteins that are isolated with these detergents are highly susceptible to contaminating peroxides and carbonyls. The peroxides will oxidize proteins and the carbonyl..
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1.5ml centrifuge tubes that are certified protein/dust free. Suitable for proteomic analysis and research. 100 tubes are supplied...
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Protein preparations specifically prepared for standardizing electrophoresis methods and protocols.
Substantially free from non-protein agents such as nucleic acids, detergents, salts, lipids and other common laboratory agents. The protein preparation has low conductivity (< 50µS..
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Protocols.io provides an interactive version of this protocol where you can discover and share optimizations with the research community.
ProteSEEKER™ identifies specific types of proteases with a panel of twelve protease inhibitors and a sensitive colorimetr..
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Gene/Protein:
An ultra sensitive and ready-to-use protein stain based on Coomassie dye. No mixing or preparation is involved. No fixation step is needed. Simply rinse gel, add RAPIDstain™ and protein bands develop within 5 to10 minutes, reaching a maximum visibility in an hour. Mini-gels can be stained in 1..
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The Reversible Copper Stain™ is a single step stain for rapid detection of proteins resolved on SDS-PAGE. No destaining is necessary.
The stain is based on the interaction of copper ions with polyacrylamide and proteins. The stain works by depositing a copper metal precipitate in th..
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The Reversible Zinc Stain™ is a single step stain for the rapid detection of proteins resolved by SDS-PAGE. No destaining is necessary.
The stain is based on the interaction of Zinc ions with polyacrylamide and proteins. The stain works by depositing a zinc metal precipitate in the ..
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Gene/Protein:
Type: Anionic detergent
Full Name: Sodium Dodecyl Sulfate
Molecular Formula: C12H25NaO4S
Molecular Weight: 288.38
Form: White to off white powder
Purity: >99%
Solubility: Water
Critical micelle concentratio..
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Gene/Protein:
A reagent kit for detection and estimation of SDS in a sample. Mix the test sample in the extraction buffer reagents provided with the kit. If SDS is present in the sample, a blue color is extracted that can be quantitatively measured.
The working range of the assay is 0.02-0.2% SDS...
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Gene/Protein:
We offer a range of secondary antibodies conjugated to either alkaline phosphatase (AP) or horseradish peroxidase (HRP). The antibodies are isolated from antisera by immuno-affinity chromatography using antigen coupled to sepharose beads or other methods. They are supplied lyophilized, from a buffer..
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SG-Arginine-C™ endopeptidase (Clostripain, from C. histolyticum) specifically hydrolyzes the carboxy peptide bond of Arginine. SG-Arginine-C™ has been modified chemically by a propriety process to render the enzyme resistant to autolysis and stabilize enzymatic activity. In addit..
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-500x500h.jpg "SG-Chymotrypsin™")
SG-Chymotrypsin™ is a serine endopeptidase, which predominantly cleaves peptide bonds on the carboxy side of tyrosine, phenylalanine and tryptophan. In addition, chymotrypsin has a low catalytic activity against the carboxy side of leucine, methionine, alanine, aspartic and glutamic acids. It ..
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SG-Glutamic-C™ is a serine endopeptidase, from S. aureus V8, that is highly specific for the cleavage of peptide bonds at the carboxy side of either aspartic or glutamic acid, depending on the buffer used. In Tris-HCl buffer, in particular in the absence of phosphate ions, the enzyme is specif..
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SG-Lysine-C™ endopeptidase, from Lysobacter enzymogenes, is a serine protease highly specific in cleaving peptide bonds at the carboxy side of lysine. Highly purified preparations of SG-Lysine-C™ are chemically modified making the enzyme resistant to autolysis and stabilizing its enzymat..
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N-Succinimidyl(4-iodoacetyl)aminobenzoate
Ideal for enzyme-antibody crosslinking
Features
Molecular Weight: 402.14
Spacer Arm (Å): 10.6
Reactive Toward: Primary Amine + Sulfhydryl
Membrane Permeable: YES
Water Soluble: NO
Cleavable/ Reversible: NO
&..
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The staining of gels with silver stains often results in over staining or unevenly stained gels. To save the trouble of repeating the electrophoresis, G-Biosciences offers Silver D-Stain™.
Features
Silver D-Stain™ can be used for reducing the staining of silver stained ge..
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A highly efficient destaining solution to remove interfering silver ions from gel pieces. Gel spots can be destained in a little as 10 minutes. Fully compatible with downstream processes, including mass spectrometry. Supplied with enough reagents for 100 protein spots...
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Succinimidyl 4-(N-maleimidomethyl) cyclohexane-1-carboxylate
Ideal for enzyme-antibody crosslinking
Features
Molecular Weight: 334.32
Spacer Arm (Å): 11.6
Reactive Toward: Primary Amine + Sulfhydryl
Membrane Permeable: YES
Water Soluble: NO
Cleavable/ Reversi..
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Succinimidyl 4-(ρ-maleimidophenyl) butyrate
Ideal for enzyme-antibody crosslinking
Features
Molecular Weight: 356.33
Spacer Arm (Å): 11.6
Reactive Toward: Primary Amine + Sulhydryl
Membrane Permeable: YES
Water Soluble: NO
Cleavable/ Reversible: NO
..
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Gene/Protein:
Features
CAS#: 1310-73-2
Molecular Formula: NaOH
Molecular Weight: 40.00
..
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%20(1)-500x500h.jpg "Stirring Balls")
Recommended for use with Micro Dialysis Cups for stirring dialysis buffer during dialysis. To achieve proper mixing of the dialysis buffer, place 5-6 stirring balls into the dialysis buffer and position on a shaker plate. When the shaker plate rotates, the stirring balls in the dialysis buffer are a..
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Protein Purification STRATEGY™ consists of numerous fractionation and chromatography techniques to aid researchers to develop a suitable purification system for specific and novel proteins. A major issue in purifying a novel protein is that the characteristics of the protein to be pur..
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N-(ε-Maleimidocaproyloxy) sulfo succinimide ester
Features
Molecular Weight: 410.33
Spacer Arm (Å): 9.4
Reactive Toward: Primary Amine + Sulfhydryl
Membrane Permeable: NO
Water Soluble: YES
Cleavable/ Reversible: NO
..
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N-γ-Maleimidobutyryloxysulfosuccinimide ester
Features
Molecular Weight: 382.38
Spacer Arm (Å): 6.8
Reactive Toward: Primary Amine + Sulfhydryl
Membrane Permeable: NO
Water Soluble: YES
Cleavable/ Reversible: NO
..
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N-Hydroxysulfosuccinimidyl-4-azidobenzoate
Features
Molecular Weight: 362.25
Spacer Arm (Å): 9.0
Reactive Toward: Primary Amines
Membrane Permeable: NO
Water Soluble: YES
Cleavable/ Reversible: NO
..
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m-Maleimidobenzoyl-N-hydroxysulfosuccinimide ester
Features
Molecular Weight: 416.30
Spacer Arm (Å): 9.9
Reactive Toward: Primary Amine + Sulfhydryl
Membrane Permeable: NO
Water Soluble: YES
Cleavable/ Reversible: NO
..
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Sulfosuccinimidyl (4-azidophenyl)-1,3’-dithiopropionate
Features
Molecular Weight: 454.44
Spacer Arm (Å): 13.9
Reactive Toward: Primary Amines
Membrane Permeable: NO
Water Soluble: YES
Cleavable/ Reversible: Reducing Agents (Thiol)
..
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Sulfosuccinimidyl 2-(m-azido-o-nitrobenzamido)-ethyl-1,3’-dithiopropionate
Features
Molecular Weight: 570.51
Spacer Arm (Å): 18.5
Reactive Toward: Primary Amines
Membrane Permeable: NO
Water Soluble: YES
Cleavable/ Reversible: Reducing Agents (Thiol)
..
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Gene/Protein:
Sulfosuccinimidyl-2-(ρ-azidosalicylamido)ethyl-1,3-dithiopropionate
Features
Molecular Weight: 541.51
Spacer Arm (Å): 18.9
Reactive Toward: Primary Amines
Membrane Permeable: NO
Water Soluble: YES
Cleavable/ Reversible: Reducing Agents (Thiol..
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N-Sulfosuccinimidyl(4-iodoacetyl)aminobenzoate
Features
Molecular Weight: 504.19
Spacer Arm (Å): 10.6
Reactive Toward: Primary Amine + Sulfhydryl
Membrane Permeable: NO
Water Soluble: YES
Cleavable/ Reversible: NO
..
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Sulfosuccinimidyl 4-(N-maleimidomethyl) cyclohexane-1-carboxylate
Ideal for enzyme-antibody crosslinking
OneQuant™ Sulfo SMCC is also available: Single use vials to minimize waste. No weighing required.
Features
Synonym: Water Soluble SMCC
CAS Number: 9292..
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Sulfo succinimidyl 4-(ρ-maleimidophenyl) butyrate
Features
Molecular Weight: 458.38
Spacer Arm (Å): 14.5
Reactive Toward: Primary Amine + Sulfhydryl
Membrane Permeable: NO
Water Soluble: YES
Cleavable/ Reversible: NO
..
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Type: Zwitterionic detergent
Full Name: N-Decyl-N,N-dimethyl-3-ammonio-1-propanesulfonate
Molecular Formula: CH3(CH2)9N+(CH3)2CH2CH2CH2SO3
Molecular Weight: 307.5
Form: White solid
Purity: >99%
Solubility: Water soluble
Critical micel..
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Type: Zwitterionic detergent
Full Name: N-Dodecyl-N,N-dimethyl-3-ammonio-1-propanesulfonate
Molecular Formula: CH3(CH2)11N+(CH3)2CH2CH2CH2SO3-
Molecular Weight: 335.5
Form: White solid
Purity: >99%
Solubility: Water soluble
Crit..
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Type: Zwitterionic detergent Full Name: N-Tetradecyl-N,N-dimethyl-3-ammonio-1-propanesulfonate Molecular Formula: CH3(CH2)13N+(CH3)2CH2CH2CH2SO3- Molecular Weight: 364.0 Form: White solid Purity: >99% Solubility: Water soluble Critica..
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-500x500h.jpg "Superior™ Blocking Buffer")
Superior™ Blocking Buffer contains a proprietary antiginically non-determinant protein for blocking non-specific sites during ELISA, membrane blotting, immunohistochemistry and other applications.
Superior™ Blocking Buffer is ideal for a high signal to background ratio in ..
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Gene/Protein:
Tissue-PE LB™ has been developed for extraction of total soluble protein from animal tissues. Tissue- PE LB™ is based on an organic buffer, which utilizes a mild non-ionic detergent, and a proprietary combination of various salts and agents to enhance extraction and stability of pr..
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Gene/Protein:
The TPE™ kit is a universal lysis system designed for the solubilization and extraction of total proteins from cells and tissues for SDS-PAGE Analysis. The kit is suitable for animal, plant, yeast, bacteria, and other biological samples.
The TPE™ kit provides a two..
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Trition® X-114
Triton® X-114 is a non-ionic detergent that is provided for research purposes or as a 10% proteomic grade solution.
Proteomic Grade: DG009, DG010, DG509, DG518
Research Grade: 786-515, 786-516
Proteomic Grade Detergent Solutions
Many commercial grade deterg..
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-500x500h.jpg "Trypsin Digestion Mix")
Gene/Protein:
The Trypsin Digestion Mix provides optimal buffered conditions for in gel trypsin digestion of proteins. Suitable for 100 protein spots.
..
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Tube-O-Array™ is specifically designed for multiple dialysis equilibrations of different samples in different buffers with Tube-O-DIALYZER™.
Tube-O-Array™ is a low cost system that allows for the rapid equilibration of samples in minimal buffer, requires minimal hands-on m..
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Tube-O-CONCENTRATOR™ is a versatile concentration device that utilizes a novel, water absorbing, liquid polymer and our patented Tube-O-DIALYZER™ for the rapid concentration of dilute protein solutions with zero protein loss. The unique tube design of Tube-O-DIALYZER™ ensures..
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Tube-O-Reactor™ is a system that allows all the key steps of cross linking, coupling and modification of proteins and/or nucleic acids to be performed in a single tube. This minimizes the risk of sample loss, of experimental time and of hands-on phases.
Most of the above reactions inv..
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Two dialysis tanks specifically designed for use with the Tube-O-DIALYZER™. Two sizes are available that are suitable for Micro and Medi size Tube-O-DIALYZER™. Each tank will accommodate 7 Tube-O-DIALYZER™ and are supplied with floats...
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Tween® 80
Tween® 80 is a non-ionic detergent that is provided for research purposes or as a 10% proteomic grade solution.
Proteomic Grade: DG013, DG014, DG513, DG520
Research Grade: 786-519, 786-520
Proteomic Grade Detergent Solutions
Many commercial grade detergents contain e..
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Gene/Protein:
Our Universal Protein Precipitation Agent, commonly known as UPPA™, has been specifically designed for the quantitative concentration of protein solutions as dilute as 1ng/ml into a small volume. Protein concentration and precipitation is not affected by the presence of detergents, chaotropics..
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Protocols.io provides an interactive version of this protocol where you can discover and share optimizations with the research community.
The UPPA-PROTEIN Concentrate™ kit uses our proprietary reagent UPPA™ (Universal Protein Precipitation Agent) to quantitatively concentr..
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Urea is a strong chemical to aid in the denaturation of proteins. Denaturants, or chaotropes, disrupt water interactions resulting in the solubilization of hydrophobic proteins and peptides. The chaotropes also act on all proteins to unfold them and alter their three-dimensional structure.
..
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Gene/Protein:
Proteomic Grade Water suitable for 2D electrophoresis and mass spectrometry analysis. Removes worries of protein and dust contamination and improves quality and reproducibility of 2D electrophoresis and mass spectrometry results.
Molecular Grade, Endotoxin-Free ..
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Western ReProbe™ is a single component system that is specifically formulated to dissociate and remove antibodies from membrane-bound proteins without destroying the antigenic binding affinity. Western ReProbe™ also enables the ability to reuse western blots.
Western ReProbe™ brea..
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