Proteomics
Our Protein Research section covers the spectrum of research products for proteomics.
There are products for Protein Purification, including Protein Extraction, Fractionation, Sample Preparation and Protein Concentration.
Our Protein Analysis products cover Protein Electrophoresis, Western Blot, and Mass Spectrometry. A wide selection of Protein Labeling and Protein Modification tools are available for protein binding and protein structure studies.
A large selection of Protein Assays are available, including unique Protein Estimation Assays.
Xpress RinseOUT™ is uniquely formulated, non-toxic and residue free cleaning spray for laboratory glassware, equipment etc. It removes proteins, fats, lipids and nucleic acid contaminants. Simply spray and rinse.
It is supplied in 16 and 32 Oz easy to apply spray bottles. The Spraying..
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Gene/Protein:
Yeast PE LB™ is useful for extraction of soluble proteins from yeast cells. Yeast PE LB™ is a proprietary improvement on the Zymolyase® based spheroplast preparation and extraction of soluble proteins from yeast cells. This kit is provided with an optional protocol to make spheroplas..
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![1-Fluoro-2,4-dinitrobenzene [DNFB]](https://cdn.gbiosciences.com/cache/DNFB-500x500h.png "1-Fluoro-2,4-dinitrobenzene [DNFB]")
1-Fluoro-2,4-dinitrobenzene [DNFB], commonly known as Sanger’s reagent is a chemical amino acid modifier that reacts with N-terminal amino acid of polypeptides. In Sanger reaction DNFB is used to form dinitrophenyl derivatives for end group determination of proteins and to derivatize primary a..
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Many commercial grade detergents contain elevated levels of sulfhydryl oxidizing agents, peroxides, salts and carbonyl compounds (Figure 1). The proteins that are isolated with these detergents are highly susceptible to contaminating peroxides and carbonyls. The peroxides will oxidize proteins and t..
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ASB-16 is a zwitterionic detergents composed of a sulfobetaine with a C16 alkyl tail that is highly efficient in solubilizing proteins for 2D electrophoresis analysis and other downstream applications. Optimal solubility is achieved in Urea and thiourea buffers, but not in urea alone.
..
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Gene/Protein:
The BCA Protein Assay, also known as the Smith Assay, is a highly sensitive colorimetric assay that is compatible with detergent solubilized protein solutions and primarily relies on two reactions. Firstly, the peptide bonds in the protein sample reduce Cu2+ ions, in a temperature dep..
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Gene/Protein:
The Micro BCA Protein Assay is a highly sensitive colorimetric assay that is compatible with detergent solubilized protein solutions. This modification of the BCA Protein Assay is suitable for dilute protein samples (0.5-20µg/ml). The Micro BCA Protein Assay primarily relies on two r..
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-500x500h.jpg "BLOT-QuickBlocker™")
Gene/Protein:
BLOT-QuickBlocker™ is a novel modified milk protein that is highly soluble and does not inhibit peroxidase detection. The modified milk protein has high blocking efficiency with a clear background.
Features
Readily soluble and produces semi-clear solution.
No inhibition ..
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G-Biosciences Carboxylation Kit With Citraconic Anhydride is designed for reversible blocking of primary amines so that directed conjugation or labeling can be performed. Citraconic anhydride reacts with primary amines and blocks them by creating an amide linkage and a terminal carboxylate. The link..
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-500x500h.jpg "CB-X™ Protein Assay")
Gene/Protein:
Protocols.io provides an interactive version of this protocol where you can discover and share optimizations with the research community.
Protein assays are routinely used in many research fields to estimate proteins in a vast array of buffers and conditions. A major pr..
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-500x500h.jpg "femtoLUCENT™ PLUS-HRP")
Gene/Protein:
femtoLUCENT™ PLUS is a femtogram level sensitive chemiluminescence immunodetection system that allows users the option to detect even hard-to-detect low abundance proteins. This immunodetection system is based on an innovative formulation of luminol and 1, 2 dioxetane, a supersensitive detecti..
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Gene/Protein:
G-Biosciences offers G-Trap™ Immobilized Amino Acids resin in a pre-packed column format. Immobilized Amino Acids are prepared by coupling amino acid to preactivated 6% cross-linked agarose. The covalent coupling to agarose (resin) is achieved by cross-linking the amino or..
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G-Trap™ Protein G are prepacked ready to use columns for affinity purification of Immunoglobulins G. The column is packed with resin, which is covalently coupled to recombinant Protein G.
Recombinant Protein G is coupled covalently by proprietary coupling method to highly crossli..
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G-Trap™ rProtein A FF are prepacked ready to use columns for purification of IgG antibodies.
The column is packed with fast flow resin, which is covalently coupled to recombinant Protein A. Recombinant Protein A is coupled covalently by proprietary coupling method to highly crosslinke..
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Gene/Protein:
Glutathione Resin is designed for the affinity purification of proteins with a glutathione S-transferase (GST) tag. The resin consists of reduced glutathione (GSH) covalently coupled to 6% cross-linked agarose, via a 10-carbon spacer arm. The resin has a binding capacity of >40mg GST/ml resin. Su..
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The amine reactive HOOK™ Activated Agarose is 6% agarose that has been activated to generate reactive aldehyde groups. The aldehyde groups of the agarose react spontaneously with primary amines, located at the N-terminus of proteins or in lysine residues, to form intermediate Schiff Base compl..
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-500x500h.jpg "Immobilized Protein A")
Gene/Protein:
Immobilized Protein A is for binding the constant domains of immunoglobulin (Ig) molecules (see Figure 1). Protein A is coupled to agarose beads by reductive amination method that provides high coupling efficiency for immunoglobulins and minimal protein A leaching (<5ng protein A/ml). Our im..
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A Solid Phase Iodination Reagent
Radioactive iodine (125I) is routinely used by researchers to label proteins. The iodination of proteins can be performed enzymatically or chemically. The Iodination reagent is designed to aid in the labeling of proteins with radioactive iodine.
The iodinat..
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Gene/Protein:
LabSafe GEL Blue™ is an enhanced protein stain based on Coomassie dye that offers unsurpassed sensitivity and rapid band visualization. LabSafe GEL Blue™ is supplied in a ready-to-use format that is added directly to protein gels following electrophoresis after a brief wash step.
&nbs..
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-500x500h.jpg "Mammalian Cell PE LB™")
Gene/Protein:
Mammalian Cell PE LB™ has been developed for extraction of total soluble proteins from mammalian cultured cells. The Mammalian Cell PE LB™ is based on organic buffering agents, which utilizes a mild non-ionic detergent, and a proprietary combination of various salts and agents to enhance..
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Gene/Protein:
For improved assay sensitivity, minimal non-specific binding, and a high signal-to-background ratio, NAP-BLOCKER™, an animal-free protein blocking agent, is recommended. NAP-BLOCKER™ offers an alternative to milk-based blocking agents, minimizing the risk of non-specific binding of antib..
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Gene/Protein:
Protocols.io provides an interactive version of this protocol where you can discover and share optimizations with the research community.
A highly sensitive, colorimetric protein assay that overcomes interference of common laboratory agents present in protein solutions and shows minimal pro..
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Nonidet® P-40 Substitute
Nonidet® P-40 Substitute is a non-ionic detergent that is provided for research purposes or as a 10% proteomic grade solution.
Proteomic Grade: DG001, DG002, DG501, DG514
Research Grade: 786-511, 786-512
Proteomic Grade Detergent Solutions
Many co..
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Gene/Protein:
The Nuclear & Cytoplasmic Extraction Kit is for the enrichment of cytoplasmic and nuclear fractions from cultured cells and tissues. The kit generates a cleaner separation of cytoplasmic proteins from nuclear proteins and is ideal for expression and transport studies.
This kit..
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-500x500h.jpg "Optimizer Buffer™")
Gene/Protein:
The conjugation and modification reactions used to cross-link proteins or couple labels to proteins, such as biotin, enzymes, and fluorescent dyes, require certain conditions, including pH and chemical composition, for optimal conjugation. Many common buffers routinely used in laboratories have an i..
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Oxidative Modification Kit is designed for the modification of glycoproteins to reactive aldehydes for amine- and hydrazide- labeling. Sodium metaperiodate, or sodium m-periodate, is a mild oxidant that is routinely used for the conversion of cis-glycol groups in carbohydrates to reactive aldehdye g..
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Gene/Protein:
A broad spectrum protease inhibitor cocktail solution that is provided as a 100X concentrated, ready-to-use solution. The cocktail contains a mix of reversible and irreversible inhibitors of serine, cysteine, calpain and metallo- proteases, including Aprotinin, PMSF, Leupeptin, Bestatin and AEBSF.&n..
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Gene/Protein:
G-Biosciences Protein Acetylation Kit is designed for modifying proteins by blocking their primary amines. The NHS ester group of Sulfo NHS Acetate reacts with primary amines of protein in non-amine containing buffers at pH 7-9. After reaction, the amine is irreversibly capped with an acyl group.
..
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Gene/Protein:
Concentration of the protein present in a sample is necessary for some laboratory protocols. Furthermore, it adds the advantage of removing other potential interfering substances like salts and detergents.
G-Biosciences Protein concentration kit ensures the highest protein recovery and is adapted f..
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Reactive Amination Kit is designed to prepare amine-reactive esters of carboxylate groups for chemical labeling. Sulfo-NHS is used to generate amine reactive esters from carboxylate groups and is primarily used to increase the efficiency of the carbodiimide EDC cross‐linking.
Features:
p..
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Gene/Protein:
Our Reducing Agent Compatible BCA Protein Assay is supplied with the Reducing Agent Compatibility Agent (RACA) that modifies reducing agents to limit their effect on the reduction of the assay’s copper ions, preventing inhibition of the assay. The use of RACA allows for samples containin..
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Gene/Protein:
A complete lysis buffer for the release of cytoplasmic, membrane and nuclear proteins from adherent and suspension cultured mammalian cells. The RIPA lysis buffer is fully compatible with many applications, including reporter assays, protein assays, immunoassays and other protein purification techni..
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SATA is used to add protected sulfhydryls to primary amines.
Kit related to SATA is also available [Sulfhydration Kit With SATA]
Features
Chemical Name: N-Succinimidyl S-acetylthioacetate
CAS Number: 76931-93-6
Molecular Weight: 245.25
Reacts primarily with primary amines
..
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Gene/Protein:
Sodium metaperiodate, or sodium m-periodate, is a mild oxidant that is routinely used for the conversion of cis-glycol groups in carbohydrates to reactive aldehdye groups (Figure 1). The reactive aldehyde groups are used in chemical conjugation procedures or detection of carbohydrates. F..
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Sulfhydration Kit With SATA is designed to introduce sulfhydryls into proteins, peptides, antibodies and other molecules which are used in conjugation and labeling of proteins.
SATA [N-Succinimidyly S-Acetylthioacetate] reagent in the kit introduces sulfhydryl groups in the proteins by reacting with..
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Sulfhydration Kit With Traut's Reagent is designed for modifying primary amine group of proteins to sulfhydryl group using Traut’s Reagent.
Traut’s Reagent or 2-Iminothiolane is a cyclic thioimidate compound that reacts with primary amines of proteins to introduce sulfhydryl groups ..
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Sulfo NHS converts carboxyl groups to amine reactive sulfo NHS esters
Kit related to Sulfo NHS is also available [Reactive Amination Kit]
Features
Chemical Name: N-Hydroxysulfosuccinimide
Reacts primarily with primary amines
Water soluble
Molecular weight: 217.13
&n..
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Sulfo NHS Acetate blocks primary amines by acylation.
Kits related to Sulfo NHS Acetate is also available [Protein Acetylation Kit]
Features
Chemical Name: Sulfosuccinimidyl acetate
Reacts primarily with primary amines
Reacts at pH 7-9
Water soluble
Molecular ..
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Gene/Protein:
Sulfo-SANPAH
N-Sulfosuccinimidyl-6-(4'-azido-2'-nitrophenylamino) hexanoate
Photoreactive Crosslinker
Sulfo-SANPAH is a water-soluble crosslinker with a long spacer-arm (18.2 angstrom) that contains an amine-reactive N-hydroxysuccinimide (NHS) ester an..
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-500x500h.jpg "Swift Membrane Stain™")
Gene/Protein:
Swift™ Membrane Stain is a unique, proprietary (patents pending), rapid, reversible, ready-to-use membrane stain for proteins on nitrocellulose or PVDF membranes. Swift™ Membrane Stain stains proteins faster and with 500 times more sensitivity than the routinely used Ponceau-S stain and ..
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Protocols.io provides an interactive version of this protocol where you can discover and share optimizations with the research community.
Western blot analysis of proteins is a routine and commonly used technique in research laboratories, with 3 major drawbacks. The first is the amount of tim..
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TCEP is a water-soluble, odorless, non-toxic and stable protein reductant. This potent reducing agent is a popular alternative to ß-mercaptoethanol and DTT (dithiothreitol) use for most applications. The reduction potency of TCEP is twice as high as that of DTT, and TCEP is effective in reduci..
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Gene/Protein:
Traut's Reagent adds sulfhydryls groups to primary amines
Kit related to Traut's Reagent is also available [Sulfhydration Kit With Traut's Reagent]
Features
Chemical Name: 2-Iminothiolane hydrochloride
Molecular weight: 137.63
Mild..
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Gene/Protein:
Trition® X-100
Triton® X-100 is a non-ionic detergent that is provided for research purposes or as a 10% proteomic grade solution.
Proteomic Grade: DG007, DG008, DG507, DG517
Research Grade: 786-513, 786-514
Proteomic Grade Detergent Solutions
Many commercia..
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Gene/Protein:
Trypsin is a serine endopeptidase that specifically cleaves peptide bonds on the carboxy side of s-aminoethyl cysteine, arginine and lysine residues. Typically there is little or no cleavage at arginyl-proline and lysyl-proline bonds.
This trypsin is purified from bovine pancreas, 1X cr..
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Gene/Protein:
A Chemically Modified, TPCK treated, Affinity Purified Trypsin. G-Biosciences' Mass Spectrometry Grade Trypsin is chemically methylated to yield an enzymatically active protein with maximum trypsin specificity and extreme resistance to autolysis. In addition, the modified trypsin is TPCK tr..
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-500x500h.jpg "Tube-O-DIALYZER™ for Laboratory Dialysis")
Gene/Protein:
Tube-O-DIALYZER™ is a unique laboratory dialysis device for small scale dialysis, 0.02-2.5ml, that allows for total sample recovery, even if protein precipitation occurs. The proprietary design is based on standard laboratory centrifuge tubes that allow common reactions, such as labeling..
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-500x500h.jpg "Tween® 20 (Polyethylene glycol sorbitan monolaurate)")
Gene/Protein:
Tween® 20 (Polyethylene glycol sorbitan monolaurate) is a non-ionic detergent that is routinely used in biochemical and life science research. G-Biosciences provides research grade Tween® 20 in 0.5L and 1L packages (786-517, 786-518) or a highly purified and sterile Prot..
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G-Biosciences Tyrosine Addition Kit is designed for conjugation of tyrosine-like residues to end-terminal α-amino groups or ε-amino groups of lysine to increase the number of tyrosyl groups that can be iodinated by iodine-125 labeling procedures. The kit is provided with Sulfo-SHP..
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Gene/Protein:
A broad range, 100X concentrated, ready-to-use protease inhibitor cocktail of AEBSF, Bestatin, E-64, EDTA, Pepstain A and PMSF that inhibits bacterial serine, cysteine and other bacterial specific proteases including aminopeptidases and aspartic proteases. The EDTA solution is provided in a spe..
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Gene/Protein:
A broad range, 100X concentrated, ready-to-use protease inhibitor cocktail of AEBSF, Aprotinin, Bestatin, E-64, EDTA, Leupeptin, Pepstatin A and PMSF that inhibits mammalian serine, cysteine and other mammalian specific proteases including aminopeptidases, trypsin-like and aspartic proteases. An EDT..
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A broad range, 100X concentrated, ready-to-use protease inhibitor cocktail of AEBSF, Bestatin, E-64, Leupeptin, Pepstatin A, 1,10-Phenathroline and PMSF that inhibits plant serine, cysteine and other plant specific proteases including aminopeptidases, aspartic and metalloproteases. This protease inh..
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Gene/Protein:
A broad range, 100X concentrated, ready-to-use protease inhibitor cocktail of AEBSF, E-64, Pepstatin A, 1,10-Phenathroline and PMSF that inhibits yeast and fungal serine, cysteine and metalloproteases. This protease inhibitor cocktail is specifically designed for the inhibition of proteases in yeast..
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A broad range, bacterial, 100X concentrated, ready-to-use protease inhibitor cocktail of AEBSF, 1,10-Phenathroline, Benzamidine, Iodoacetamide, Pepstain A and PMSF that offers greater protection for recombinant proteins expressed and purified from bacteria. The protease inhibitor cocktail inhibits b..
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Gene/Protein:
FOCUS™ ProteaseArrest™ is a ready-to-use, 100X concentrated, broad range protease inhibitor cocktail that is fully compatible with 2D electrophoresis and subsequent mass spectrometry. The protease inhibitor cocktail consits of AEBSF, Aprotinin, Bestatin, Calpain Inhibitor I, Leupeptin, P..
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-500x500h.jpg "ProteCEASE™")
Gene/Protein:
ProteCEASE™ is a dry format version of our ProteaseArrest™ for large scale preparative applications and for those who prefer reconstitution prior to use.
ProteCEASE™ is a superior general protease inhibitor cocktail that is suitable for purification from mamm..
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Gene/Protein:
Contains 12 ready-to-use individual protease inhibitors for characterization of protease activity.
Each set contains the following protease inhibitors. See individual inhibitors for their specificities and other information.:
AEBSF
ALLN
Antipain, dihydrochloride..
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4-(2-Aminoethyl) benzenesulfonyl fluoride hydrochloride
Specificity: Specific irreversible inhibitor of serine proteases, including chymotrypsin, kallikrein, plasmin, thrombin and trypsin. A stable non-toxic alternative to PMSF.
Solubility: Soluble in water
Features
Sy..
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Calpain inhibitor I; N-[N-(N-Acetyl-L-leucyl)-L-leucyl]-L-norleucine
Specificity: Cell permeable peptide aldehyde inhibitor of calpain I and to a lesser extent calpain II. Also inhibits other neutral cysteine proteases, cathepsin B and L and the proteasome.
Solubility: Solubl..
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Gene/Protein:
[(S)-1-Carboxy-2-Phenyl]-carbamoyl-Arg-Val-arginal
Specificity: Inhibits Ca2+-dependent endopeptidases, including papain, trypsin-like serine proteases, some cysteine proteases and to a lesser extent plasmin. Higher specificity for trypsin and papain compared to leupeptin...
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Gene/Protein:
Also known as bovine pancreatic trypsin inhibitor.
Activity: Approx 6,000 KIU/mg
Specificity: A broad range, competitive and reversible inhibitor of chymotrypsin, plasmin, trypsin, kallikrein and other serine proteases
Solubility: Soluble ..
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N-[(2S,3R)-3-Amino-2-hydroxy-4-phenylbutyryl]-L-leucine hydrochloride
Specificity: Competitive inhibitor of surface aminopeptidases, including aminopeptidase B (Ki=2nM), leucine aminopeptidase (Ki=20nM). Also inhibits aminopeptidases N; does not inhibit endoproteases.
..
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N-[(S)-1-carboxy-isopentyl)-carbamoyl-alpha-(2-iminohexahydro-4(S)-pyrimidyl]-L-glycyl-L-phenylalaninal
Specificity: Inhibits serine proteases having a chymotrypsin-like specificity, including α, β γ, and δ chymotrypsin, and most cysteine proteases including ca..
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E-64
L-trans-epoxysuccinyl-leucylamide-(4-guanido)-butane or N-[N-(L-trans-carboxyoxiran-2-carbonyl)-L-leucyl]-agmatine
Features
CAS Number: 66701-25-5
Molecular Formula : Câ‚â‚…H₂₇Nâ‚…Oâ..
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Gene/Protein:
EDTA disodium salt is a reversible metalloprotease inhibitor that is used for chelating metal ions. A 0.5M solution is also available.
Chemical Name: EDTA (Ethylenediaminetetracetic acid)
Synonyms: disodium ethylenediaminetetraacetate dihydrate; ethylenediamine tetraace..
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Gene/Protein:
Features
Specificity: Inhibits serine, plasmin, porcine kallikrein and cysteine proteases, including papain and cathepsin B. Does not inhibit chymotrypsin and thrombin.
Molecular weight: 475.59
Purity: Three tautomeric isomers in solution
Solubility: Soluble in H2O (5..
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Gene/Protein:
Isovaleryl-Val-Val-AHMHA-Ala-AHMHA where AHMHA= (3S, 4S)-4-amino-3-hydroxy-6-methyl-heptanoic acid
Specificity: A potent inhibitor of various aspartic proteases, including cathepsin D, renin, pepsin, bacterial aspartic proteases and HIV proteases.
Solubility: Soluble in DMS..
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N-alpha-L-rhamnopyranosyloxy(hydroxyphosphinyl)-L-Leucyl-L-Tryptophan
Specificity: Inhibits some metalloproteases, including thermolysin, collagenase and bacterial metallo proteases from Bacillus subtilis, Streptomyces griseus and Pseudomonas aeruginosa (metallo elastase).
Solubili..
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Features
Synonyms: α-Toluenesulfonyl fluoride, Benzylsulfonyl fluoride, Phenylmethylsulfonyl fluoride
CAS Number: 329-98-6
Molecular Formula: C₇H₇FO₂S
Molecular Weight: 174.19 g/mol
Activity: >99.9%
Solubility: Soluble in..
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