Proteomics
Our Protein Research section covers the spectrum of research products for proteomics.
There are products for Protein Purification, including Protein Extraction, Fractionation, Sample Preparation and Protein Concentration.
Our Protein Analysis products cover Protein Electrophoresis, Western Blot, and Mass Spectrometry. A wide selection of Protein Labeling and Protein Modification tools are available for protein binding and protein structure studies.
A large selection of Protein Assays are available, including unique Protein Estimation Assays.
FOCUS™ Global Fractionation kit is designed to fractionate complex biological samples into cytosolic and membrane fractions. The resulting membrane fractions are subsequently fractionated into either peripheral and integral membrane proteins or lipid raft associated proteins and detergent solu..
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FOCUS™ Glycoprotein kit is based on lectin binding of specific glycoproteins with terminal α-D mannosyl and α-D glycosyl proteins.
FOCUS™ Glycoprotein kit isolates glycoproteins from complex biological solutions using spin columns that contain lectin (Concanavalin A)..
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Extracts and solubilizes nearly all of the proteins from insect cell cultures (i.e. Sf9 and Sf21), including membrane as well as soluble proteins, using a strong chaotropic extraction buffer to solubilize even the most difficult proteins.
Features
Single step extraction protoco..
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Gene/Protein:
Extracts and solubilizes nearly all of the proteins from mammalian samples, including membrane as well as soluble proteins, by a strong chaotropic extraction buffer to solubilize even the most difficult proteins. Suitable for biological samples from animal tissues and adherent and suspension c..
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Gene/Protein:
FOCUS™ Membrane Proteins is a rapid and highly reproducible method for preparation of membrane or hydrophobic proteins from biological samples for 2D-gel analysis or other applications. Membrane proteins are extracted with a single step phase partition, with an efficiency greater than 90% with..
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Protocols.io provides an interactive version of this protocol where you can discover and share optimizations with the research community.
Specifically designed for the isolation of intact mitochondria from cultured mammalian cells. This kit allows for the fast and efficient fractionation of t..
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FOCUS™ PhosphoRich™ is a ready-to-use kit that enriches phosphorylated proteins and phosphopeptides from complex biological samples. The kit contains spin columns that have a phosphoprotein binding resin with a binding capacity of ~20mg phosphorylated ovalbumin per column. The resin colu..
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Specifically designed for plant research and supplied with plant specific reagents, including reagents for removal of plant pigments and other natural products that may interfere with protein analysis. Extracts and solubilizes nearly all of the proteins from plants, including membrane as well as sol..
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The kit is supplied with a proprietary buffer necessary for efficient alkylation of thiols, while minimizing reoxidation of the competing thiol pairs in protein samples. Simply add an appropriate amount to reagent solutions for alkylation of protein thiol groups. Features Pro..
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A water soluble, odorless, non-toxic and stable TCEP [Tris (2-carboxyethyl) phosphine] reductant for protein reduction.
FOCUS™ Protein Reductant has improved stability and efficiency compared to DTT and reduces proteins over a wide range of pH, including lower acidic pH. FOCUS™ ..
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The FOCUS™ Reduction-Alkylation kit offers a simple two-step method for reduction and alkylation of protein samples for 2D gel analysis. The disulfide bonds are reduced with a highly reactive and stable TCEP [Tris (2-carboxyethyl) phosphine] followed by blocking of the thiols by alkyl..
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Gene/Protein:
Lipid rafts are membrane microdomains that are enriched in caveolin, cholesterol, glycolipids, sphingolipids and glycosyl-phosphatidylinositol. Lipid rafts are also known as detergent-insoluble glycolipid-enriched complexes (GEMs) or DIGs. Many signaling proteins, including glycosylphosphatidylinosi..
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