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Cross-Linking & Modification

Download the Crosslinkers Handbook & Selection Guide            Download the Protein Labeling & Conjugation Handbook

 

 Cross-linking agents contain at least two reactive groups that are reactive towards numerous groups, including sulfhydryls, amines and carbohydrates, and create chemical covalent bonds between two or more molecules.  functional groups that can be targeted with cross-linking agents are primary amines, carboxyls, sulfhydryls,  carbohydrates and carboxylic acids. Protein molecules have many of these functional groups and therefore proteins and peptides can be readily conjugated using cross-linking agents. Cross-linking agents are used to study protein structure and function, to anchor proteins to solid  supports, preparation of immunogens, immunotoxins, and other conjugated protein reagents. G-Biosciences offers a variety of Double Do™ cross-linking agents, reaction specific buffers, and accessories for performing and facilitating crosslinking applications. These accessory tools simplify cross-linking reactions and will enable researchers to achieve highly efficient cross-linking reactions with minimal effort in reaction optimization, set-up, and procedure development

 
We offer a variety of amino acid side chain modifiers to block side chain interactions, change the charge or modify them to be more favorable for other reactions. Many denaturants, or chaotropes, are available and can disrupt water interactions resulting in the solubilization of hydrophobic proteins and peptides. Chaotropes act on proteins to unfold them and alter their three-dimensional structure. A wide selection of reagents and accessories are also available.
The conjugation and modification reactions used to cross-link proteins or couple labels to proteins, such as biotin, enzymes, and fluorescent dyes, require certain conditions, including pH and chemical composition, for optimal conjugation. Many common buffers routinely used in laboratories have an i..
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HPG (p-hydroxyphenylglyoxal) reacts to specifically modify arginine residues under mild conditions to yield spectrophotometrically measurable signal for amino acid detection.   Features of HPG: • Arginine-specific—reacts specifically with arginine residues under mild conditions (p..
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N-(ρ-Maleimidophenyl) isocyanate   Features Molecular Weight: 214.18 Spacer Arm (Å): 8.7 Reactive Toward: Sulfhydryl + Hydroxyl Membrane Permeable: Not tested  Water Soluble: NO Cleavable/ Reversible: NO    ..
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A water soluble, odorless, non-toxic and stable protein reductant. Protein-S-S-Reductant™ uses TCEP (Tris [2-carboxyethyl] phosphine), a popular alternative to ß-mercaptoethanol and DTT (dithiothreitol). TCEP improves stability, increases effectiveness, and reduces proteins over a wider ..
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  Features Chemical Name: N-Succinimidyl S-acetylthioacetate CAS Number: 76931-93-6 Molecular Weight: 245.25 Reacts primarily with primary amines Adds protected sulfhydryl residues Sulfhydryl group can be used in coupling reactions Soluble in DMSO Chemi..
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  SG-Arginine-C™ endopeptidase (Clostripain, from C. histolyticum) specifically hydrolyzes the carboxy peptide bond of Arginine. SG-Arginine-C™ has been modified chemically by a propriety process to render the enzyme resistant to autolysis and stabilize enzymatic activity. In addit..
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SG-Chymotrypsin™ is a serine endopeptidase, which predominantly cleaves peptide bonds on the carboxy side of tyrosine, phenylalanine and tryptophan. In addition, chymotrypsin has a low catalytic activity against the carboxy side of leucine, methionine, alanine, aspartic and glutamic acids. It ..
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SG-Glutamic-C™ is a serine endopeptidase, from S. aureus V8, that is highly specific for the cleavage of peptide bonds at the carboxy side of either aspartic or glutamic acid, depending on the buffer used. In Tris-HCl buffer, in particular in the absence of phosphate ions, the enzyme is specif..
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SG-Lysine-C™ endopeptidase, from Lysobacter enzymogenes, is a serine protease highly specific in cleaving peptide bonds at the carboxy side of lysine. Highly purified preparations of SG-Lysine-C™ are chemically modified making the enzyme resistant to autolysis and stabilizing its enzymat..
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N-Succinimidyl(4-iodoacetyl)aminobenzoate   Ideal for enzyme-antibody crosslinking   Features Molecular Weight: 402.14 Spacer Arm (Å): 10.6 Reactive Toward: Primary Amine + Sulfhydryl Membrane Permeable: YES Water Soluble: NO Cleavable/ Reversible: NO   &..
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Succinimidyl 4-(N-maleimidomethyl) cyclohexane-1-carboxylate   Ideal for enzyme-antibody crosslinking   Features Molecular Weight: 334.32 Spacer Arm (Å): 11.6 Reactive Toward: Primary Amine + Sulfhydryl Membrane Permeable: YES Water Soluble: NO Cleavable/ Reversi..
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Succinimidyl 4-(ρ-maleimidophenyl) butyrate   Ideal for enzyme-antibody crosslinking   Features Molecular Weight: 356.33 Spacer Arm (Å): 11.6 Reactive Toward: Primary Amine + Sulhydryl  Membrane Permeable: YES Water Soluble: NO Cleavable/ Reversible: NO ..
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