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Purification & Chromatography

Download the Protein Purification Handbook for a full list of related productsDownload the Purification Resins Handbook for Complete Product List

 

We offer a large selection of products for protein purification and chromatography.

Protein Purification STRATEGY™ consists of numerous fractionation and chromatography techniques including pH fractionation, salt fractionation, hydrophobic chromatography, anionic chromatography, and cationic chromatography to aid researchers in developing suitable purification systems for specific and novel proteins.

Our G-Capsule is an easy-to-use device for the rapid electroelution of nucleic acids and proteins that excises DNA or protein bands and elutes your sample in a final volume of ~30µl with as high as 90% protein recovery.  

We offer many products to aid in affinity chromatography for the separation & purification of affinity tagged proteins & antibodies. Immobilized Protein A is available for the purification of immunoglobulin (ig) molecules and Immobilized Protein G is offered for the purification of antibodies through the constant IG domains. Our nickel chelating resin is an immobilized metal affinity chromatography (IMAC) resin that utilizes nickel for the purification of 6x histidine tagged proteins.

Epoxy-Activated Agarose is preactivated resin used for affinity chromatography. Epoxy-Activated Agarose comprise of high density epoxy groups that form covalent bonds with ligands containing amine groups, thiol or hydroxyl groups.   Epoxide-Acivated Agarose is prepared by immobilization of ox..
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Electroelution of nucleic acids and proteins has many advantages as it avoids centrifugation, vortexing, heating, precipitation and allows minimal manipulation of samples. Electroelution normally involves dialysis tubing, which results in extreme dilution of precious samples. G-Capsule™ is a s..
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G-Sep™ is a gel filtration resin comprised of ultrapure cross-linked dextran for desalting and buffer exchange in industrial applications. It exhibits high selectivity, superb resolution, low non-specific adsorption and robust chemical stability. Quickly desalts, removes contaminants and tr..
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G-Sep™ Agarose 4B is a gel filtration matrix formed from agarose beads that is available with 4% agarose content, designated G-Sep™ Agarose 4B.   The resin is a proven gel filtration base matrix and is routinely modified by researchers to couple affinity ligands.  The resin i..
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G-Sep™ Agarose 6B is a gel filtration matrix formed from agarose beads that is available with 6% agarose content, designated G-Sep™ Agarose 6B.   The resin is a proven gel filtration base matrix and is routinely modified by researchers to couple affinity ligands.  The resin i..
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G-Sep™ Agarose CL-4B resin is a cross-linked versions of our G-Sep™ Agarose. Cross-linking of the agarose results in chemically and physically more stable agarose beads that offer the same selectivity, but with better flow characteristics. Cross-linked agarose beads are resistant to..
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G-Sep™ Agarose CL-6B resin is a cross-linked versions of our G-Sep™ Agarose.  Cross-linking of the agarose results in chemically and physically more stable agarose beads that offer the same selectivity, but with better flow characteristics. Cross-linked agarose beads are r..
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G-Sep™ Agarose 6 Fast Flow (FF) is a gel filtration matrix formed from agarose beads that is based on our cross-linked 6% agarose.    The modification to the cross-linked 6% agarose to fast flow results in improved physical stability and chromatographic qualities.  The modific..
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G-Sep™ Butyl Agarose Fast Flow (FF) is a 6% cross-linked agarose with butyl groups for Hydrophobic Interaction Chromatography (HIC).  The resin is used to separate biomolecules on the basis of relative hydrophobicity.     G-Sep™ Agaroses for HIC are also available with o..
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G-Sep™ CM Agarose Fast Flow (FF) resin is a weak cation exchanger composed of highly cross-linked 6% agarose beads, with Carboxymethyl (CM) weak cation exchange groups.   The resin is extensively used for biomacromolecule separations in both research and industrial applications. The G-S..
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Immobilized Metal Ion Affinity Chromatography (IMAC), developed by Porath (1975), is based on the interaction of certain protein residues (histidines, cysteines, and to some extent tryptophans) with cations of transition metals.  G-Sep™ Co IDA Agarose Fast Flow is specifically designed fo..
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G-Sep™ DEAE Agarose Fast Flow (FF) resin is a weak anion exchanger composed of highly cross-linked 6% agarose beads, with diethylaminoethyl (DEAE) weak anion exchange groups.   The resin is extensively used for biomacromolecule separations in both research and industrial applications. T..
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