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Purification & Chromatography

G-Biosciences offers protein purification and protein chromatography reagents, including agarose resins for coupling researchers ligands, via versus coupling chemistries, or pre-coupled ligands for purification of specific protein tags. We offer activated agarose resins for conjugation of proteins and biomolecules through amines, carbohydrates, carboxyls, sulfhydryls, hydroxyls and activated hydrogens for coupling your ligand to agarose or magnetic beads. We offer premade protein purification resins for the isolation of specific protein groups or protein tags, including streptavidin and avidin purification, biotinylated protein purification, calmodulin binding protein (CBP) purification, C-Reactive protein purification, GST tagged protein purification, 6X His tagged protein purification, Heparin binding protein purificaiton and glycoprotein purification with immobilized lectins. For antibody purification resins, Protein A resin, Protein G resin and Protein A/G resin are offered in multiple formats, including resin, spin columns and FPLC columns.  Several antibody purification products based on our proprietary Pearl™ IgG Purification resin are offered, including an antibody clean-up kit and a monoclonal antibody purification kit.

Gel filtration resins or size exclusion resins are also offered as resin alone or as spin format columns. The resins offered are G-Sep™-25 and G-Sep™-50 that are equivalents to the well established gel filtration resins for desalting and buffer exchange.  Agarose 4B, CL-4B, 6B and CL-6B are agarose and cross-linked agarose size exclusion chromatography base matrix that are frequently used for coupling affinity ligands to the matrix. Theses matrixes are not pre-activated and the user performs all steps in coupling. The columns available are our cross-linked dextran resin SpinOUT™ columns or the polyacrylamide SpinOUT™ G-Acryl columns for rapid desalting or buffer exchange applications and our prefilled size exclusion columns.

Hydrophobic Interaction Chromatography (HIC) resins, including butyl agarose, octyl agarose and phenyl agarose are offered as resin alone or in 1 and 5ml FPLC columns. A convenient HIC selection kit  featuring prepacked 1 ml columns of G-Trap™ Butyl Agarose 6 Fast Flow, G-Trap™ Octyl Agarose 6 Fast Flow, G-Trap™ Phenyl Agarose 6 Fast Flow (High Sub) and G-Trap™ Phenyl Agarose 6 Fast Flow (Low Sub) is also available.

Ion Exchange Chromatography resins, including the cation exchanger resins CM agarose and SP agarose and the anionic exchanger resins DEAE agarose and Q agarose. These resins are offered as G-Trap™ FPLC columns, including a G-Trap™ Ion Exchange Selection kit, featuring a 1ml columns of G-Trap™ CM Agarose Fast FlowG-Trap™ SP Agarose Fast FlowG-Trap™ DEAE Agarose Fast Flow and G-Trap™ Q Agarose Fast Flow column.

Empty FPLC columns, empty low pressure glass columns, empty spin columns and empty gravity flow columns are available for researchers to pack their own columns or researchers can utilize G-Biosciences column packing service.

For small scale protein purification of specific proteins and protein complexes, four immunoprecipitation kits are offered. The Classic Immunoprecipitation, a Co-Immunoprecipitation, a Cross-link Immunoprecipitation and a Direct Immunoprecipitation.

 

Download the Protein Purification Handbook for a full list of related products

Download the Purification Resins Handbook for Complete Product List

L-Threonine  is an essential α-amino acid  which is used in the biosynthesis of proteins. Immobilized L-Threonine is prepared by covalently coupling amiono group of L-threonineto the preactivated 6% cross-linked agarose.   ​Click here to request bulk or custom sizes. Features:..
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 L-Tryptophan is an essential α-amino acid  which is used in the biosynthesis of proteins. Immobilized  L-Tryptophan is prepared by covalently coupling amiono group of  L-tryptophan to the preactivated 6% cross-linked agarose.   ​Click here to request bulk or c..
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L-Tyrosine  is an α-amino acid  which is used in the biosynthesis of proteins. Immobilized L-Tyrosine is prepared by covalently coupling amiono group of L-tyrosine to the preactivated 6% cross-linked agarose. ​ Click here to request bulk or custom sizes. Features: L-Tyrosine ..
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L-Valine  is an essential α-amino acid  which is used in the biosynthesis of proteins. Immobilized L-Valine is prepared by covalently coupling amiono group of L-valine to the preactivated 6% cross-linked agarose. ​Click here to request bulk or custom sizes.   Features: L..
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G-Biosciences Immobilized Monomeric Avidin Resin is designed for the simple affinity chromatography purifications of proteins, antibodies and other molecules with a biotin tag.  The resin consists of monomeric subunits of avidin covalently coupled to 6% cross-linked agarose, offering a stable, ..
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Neomycin is a bactericidal aminoglycoside antibiotic. It binds to the 30S ribosome of susceptible organisms and interferes with mRNA binding and acceptor tRNA sites and results in the production of non-functional or toxic peptides. Immobilized Neomycin is prepared by covalently coupling neomycin to..
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Penicillin G is a naturally occurring beta lactam antibiotic produced by the Penicillium fungus. Penicillin G works by inhibiting cell wall production in non-resistant cells by breaking the links between peptidogylocans and causing the production of cell wall damaging autolysins. Immobilized ..
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Immobilized Procainamide is designed for affinity purification of butyrylcholinesterase (BuChE), which is also known as non-specific cholinesterase. Butyrylcholinesterase can be purified by batch adsorption on immobilized Procainamide followed by ion-exchange chromatography on DEAE-Sepharose column..
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Immobilized protein G is for binding the constant domains of immunoglobulin (Ig) molecules (see Figure 1). It is ideal for the easy one-step purification of classes, subclasses and fragments of immunoglobulins for biological fluids and cell culture media. Protein G is a modified form of Streptococca..
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Biotin, a 244Da vitamin (Vitamin H) molecule, exhibits an extraordinary binding affinity for avidin (Ka=10¹âµM-¹) and streptavidin (Ka=10¹âµM-¹). Biotin and (strept)avidin interaction is rapid. Once the bond is established, it can survive up to 3M guanid..
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Streptomycin Sulfate is an aminoglycoside antibiotic and has a bactericidal action against many gram-negative bacteria. It binds to the S12 protein on the ribosomal 30S subunit and inhibits protein synthesis    Immobilized Streptomycin is made by covalentlly coupling streptomycin to..
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Sulfadimidine is a bacteriostatic antimicrobial sulfur drug. It blocks the synthesis of dihydrofolic acid by inhibiting the enzyme dihydropteroate synthase. Sulfadimidine is a competitive inhibitor of bacterial para-aminobenzoic acid (PABA), which is required for bacterial synthesis of folic acid. &..
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